A new black Aspergillus species, A. vadensis, is a promising host for homologous and heterologous protein production

Applied and Environmental Microbiology
Ronald P de VriesJaap Visser

Abstract

A new species of the group of black aspergilli, Aspergillus vadensis, was analyzed for its potential as a host for homologous and heterologous protein production. Unlike the other black aspergilli, this strain does not acidify the culture medium when nitrate is the nitrogen source and only produces very low levels of extracellular proteases, mainly serine metalloproteases. The stability of A. tubingensis feruloyl esterase A (FaeA) was compared upon production in wild-type A. vadensis, A. tubingensis, and an A. niger strain in which the three main protease-encoding genes were disrupted. The production of FaeA in A. vadensis resulted in larger amounts of intact protein than production in A. tubingensis and was similar to production in an A. niger protease disruptant, confirming in vivo the low proteolytic activity of A. vadensis. The protoplast formation and transformation efficiencies of A. vadensis were much higher than those of A. niger. These characteristics make A. vadensis a very promising candidate for homologous, and possibly heterologous, protein production.

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Citations

Dec 13, 2007·Applied Microbiology and Biotechnology·Qiang LiBrian McNeil
Apr 17, 2008·Applied Microbiology and Biotechnology·Maria MoukouliPaul Christakopoulos
Jun 10, 2010·Applied Microbiology and Biotechnology·André Fleissner, Petra Dersch
Apr 2, 2005·Antonie van Leeuwenhoek·Ronald P de VriesJaap Visser
Mar 27, 2012·Journal of Proteome Research·Pauline KrijgsheldHan A B Wösten
Dec 18, 2012·Applied and Environmental Microbiology·Joost van den BrinkRonald P de Vries
Oct 24, 2007·Eukaryotic Cell·Ana M LevinHan A B Wösten
Mar 25, 2009·BMC Microbiology·Mayken W WadmanJohannes F G Vliegenthart
Aug 6, 2011·Genome Biology·Charissa de BekkerHan A B Wösten
Jan 10, 2008·Antonie van Leeuwenhoek·Ronald P de VriesJoost A Stalpers
Mar 19, 2014·Bioresource Technology·Radhika Deshmukh, Hemant J Purohit
Apr 13, 2011·Applied Microbiology and Biotechnology·Evy BattagliaRonald P de Vries
Apr 1, 2014·Applied Microbiology and Biotechnology·Birgit S GrubenRonald P de Vries
Oct 16, 2008·Archives of Microbiology·Miroslava DuranováRonald P de Vries
Sep 28, 2014·Journal of Industrial Microbiology & Biotechnology·Helena CulletonRonald P de Vries
Feb 18, 2016·Biotechnology Letters·Aleksandrina PatyshakuliyevaIsabelle Benoit Gelber
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Feb 2, 2017·Microbial Cell Factories·Emma KarlssonLisbeth Olsson

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