A new C-type animal lectin isolated from Bothrops pirajai is responsible for the snake venom major effects in the isolated kidney

The International Journal of Biochemistry & Cell Biology
A HavtH S A Monteiro

Abstract

We investigated the biochemical and biological effects of a new C-type galactoside specific lectin termed BPL that was isolated from the snake venom of Bothrops pirajai. This lectin was purified using size exclusion HPLC followed by an immobilized lactose affinity column. The purified BPL was homogeneous by reverse phase HPLC and SDS-PAGE. We evaluated the nephrotoxicity of the whole venom of B. pirajai and its lectin. The whole venom of B. pirajai (10 microg/mL) showed similar results as those observed for BPL (3, 10 and 30 microg/mL) evaluated by the perfused rat kidney method. They caused reductions in perfusion pressure (Control120 = 110.28 +/- 3.69; BP120 = 70.70 +/- 2.40*; BPL3(120) = 113.20 +/- 4.40; BPL10(120) = 67.80 +/- 3.00*; BPL30(120) = 64.90 +/- 3.50* mmHg; *: P < 0.05), renal vascular resistance, urinary flow, glomerular filtration rate (Control90 = 0.695 +/- 0.074; BP90 = 0.142 +/- 0.032*; BPL3(90) = 0.314 +/- 0.064; BPL10(90) = 0.250 +/- 0.038*; BPL30(90) = 0.088 +/- 0.021* mLg(-1) min(-1); *: P < 0.05) and sodium (Control120 = 81.28 +/- 0.26; BP120 = 55.71 +/- 5.72*; BPL3(120) = 80.94 +/- 0.93; BPL10(120) = 65.23 +/- 1.47*; BPL30(120) = 76.03 +/- 1.70* %; *: P < 0.05), potassium and chloride tubular transport....Continue Reading

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Citations

Jun 12, 2010·Toxicon : Official Journal of the International Society on Toxinology·Ji-Fu WeiShao-Heng He
Dec 29, 2009·Toxicon : Official Journal of the International Society on Toxinology·Inez Liberato EvangelistaHelena Serra Azul Monteiro
Oct 13, 2009·Toxicon : Official Journal of the International Society on Toxinology·Sueli M MelloStephen Hyslop
Apr 28, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ji-Fu WeiShao-Heng He
Oct 19, 2006·Toxicon : Official Journal of the International Society on Toxinology·Paula Alvarez AbreuHelena Carla Castro
May 30, 2006·Toxicon : Official Journal of the International Society on Toxinology·Marcus Davis Machado BragaHelena Serra Azul Monteiro
Nov 29, 2005·Toxicon : Official Journal of the International Society on Toxinology·Patrícia C PanuntoStephen Hyslop
Sep 29, 2015·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Marco A Sartim, Suely V Sampaio
Apr 11, 2014·BioMed Research International·Kayena D ZaqueoRodrigo G Stábeli
Nov 28, 2012·International Journal of Biological Macromolecules·Letícia Eulálio CastanheiraVeridiana M Rodrigues
Feb 22, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Erika dos Santos NunesMaria Tereza dos Santos Correia
Dec 3, 2011·Toxicon : Official Journal of the International Society on Toxinology·Adolfo Rafael de RoodtRoberto Pablo Stock
Feb 8, 2011·Acta Biochimica Et Biophysica Sinica·Elaine de Paula Mendonça-FranqueiroSuely Vilela Sampaio
May 13, 2014·Surgical Neurology International·David StrausVincent C Traynelis
Dec 12, 2020·Toxicon : Official Journal of the International Society on Toxinology·Aline Diogo MarinhoHelena Serra Azul Monteiro
Jun 6, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·S G De-SimoneF P Silva
May 7, 2020·Journal of Proteome Research·Rongfeng LiPengcheng Li

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