PMID: 8601577Jan 1, 1996Paper

A new cell line from human infiltrating ductal carcinoma of the breast: establishment and characterization

Journal of Cancer Research and Clinical Oncology
W ZoliA Gasperi-Campani

Abstract

We established a novel cancer cell line (MAST) from the ascitic fluid of a metastatic infiltrating ductal carcinoma of the breast. The epithelial and neoplastic nature of the MAST cells was confirmed by ultrastructural analysis. The cell line was maintained as a monolayer with a doubling time of about 68 h, and it possessed an abnormal karyotype with a modal chromosome number of 60, a trisomy of chromosome 18 and other unidentified rearranged chromosomes. Among the markers consistently found in MAST metaphases, we noted a t(14; 14) and a very large subtelocentric, a large satellited acrocentric and a very large submetacentric chromosome with striking fluorescent bands. Immunoenzymatic assay demonstrated that the MAST cell line was positive for estrogen and progesterone receptors. The in vitro drug-sensitivity assay showed a marked resistance of the cell line to 5-fluorouracil and 4-hydroperoxycyclophosphamide and a moderate resistance to etoposide and 4'-epidoxorubicin. The molecular analysis showed a four-to sixfold amplification of the c-myc gene and no amplification or rearrangement of the int-2, c-erbB-2, c-Ha-ras, c-mos and hst-1 genes.

References

Jan 1, 1975·Advances in Human Genetics·B Dutrillaux, J Lejeune
Aug 1, 1989·British Journal of Cancer·S MinafraD E Woolley
Jun 1, 1989·Current Opinion in Immunology·R Kath, M Herlyn
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M TairaT Sugimura
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R WatsonG F Vande Woude
Jan 1, 1982·Journal of Cellular Biochemistry·S PulcianiM Barbacid
Dec 30, 1994·Biochimica Et Biophysica Acta·P Devilee, C J Cornelisse
Dec 1, 1993·Breast Cancer Research and Treatment·D AmadoriW Zoli
Jan 1, 1993·Advances in Cancer Research·M J van de Vijver

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Citations

Aug 5, 1998·Cancer Genetics and Cytogenetics·W ZoliA Gasperi-Campani
Nov 11, 1998·Cancer Genetics and Cytogenetics·A Gasperi-CampaniD Amadori
Aug 26, 1998·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·A Gasperi-CampaniD Amadori
Apr 1, 1997·Breast Cancer Research and Treatment·W ZoliA Gasperi-Campani
Feb 6, 2004·Breast Cancer Research and Treatment·Marc Lacroix, Guy Leclercq

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