PMID: 6402011Jan 5, 1983Paper

A new class of inhibitors for in vitro small intestinal transport of sugars and amino acids in the rat

Biochimica Et Biophysica Acta
B ElsenhansW F Caspary

Abstract

Polycationic compounds like polylysine, protamine or polyethylenimine may interfere with a cation-related membrane transport system depending on superficially accessible binding sites for particular cations. In vitro experiments were performed using either everted segments of rat small intestine to measure tissue accumulation or everted sacs to determine mucosal-to-serosal transport. The effect of polycations was also tested using brush-border membrane vesicles of rat jejunum. Polycations inhibited the tissue accumulation of methyl alpha-D-glucoside as well as binding of phlorizin. Inhibition of accumulation was increased by raising the polycation concentration and by preincubation of the tissue with the polycations. Kinetic experiments revealed a competitive type of inhibition for the uptake of neutral amino acids and actively transported sugars. Using everted sacs to compare the monomeric cations with their corresponding polymeric forms for their inhibitory effect, it was found that only polymers applied to the mucosal compartment impaired active transport. The passive diffusion of solutes, e.g. 2-deoxy-D-glucose or mannitol, was slightly increased by polycations. With some intermediate oligomers of lysine it could be shown t...Continue Reading

References

Feb 4, 1977·Deutsche medizinische Wochenschrift·W F Caspary
Mar 20, 1978·Pflügers Archiv : European journal of physiology·H LúckeH Murer
Jul 1, 1978·The American Journal of Anatomy·R A Jersild, R W Crawford
Aug 1, 1975·The American Journal of Physiology·J J HajjarA B Bikhazi
Sep 1, 1969·The American Journal of the Medical Sciences·W O Dobbins
Sep 27, 1973·Biochimica Et Biophysica Acta·J SchmitzR K Crane
Jul 1, 1972·Biochemical Pharmacology·J D Teale, A H Love
Aug 1, 1971·Journal of Bioenergetics·A Huunan-Seppälä
May 1, 1966·The Journal of General Physiology·S G SchultzP F Curran
Apr 1, 1967·Biochemistry·C L JohnsonA Schwartz
May 1, 1967·The Journal of General Physiology·P F CurranR E Fuisz
Dec 18, 1960·Biochimica Et Biophysica Acta·R K CRANE, P MANDELSTAM

❮ Previous
Next ❯

Citations

Jul 9, 2002·Biomaterials·Chris Zhisheng Chen, Stuart L Cooper
Feb 28, 1985·Biochimica Et Biophysica Acta·B ElsenhansW F Caspary
Jan 1, 2000·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·P K CheluleM F Dutton
Mar 8, 1995·Biochimica Et Biophysica Acta·H P SchedlG R Buettner
Mar 1, 1995·Medicinal Research Reviews·K M HillgrenR T Borchardt
Jul 1, 1984·British Journal of Pharmacology·E S Debnam, C S Thompson
Jan 1, 1987·Radiation and Environmental Biophysics·P Marszałek, A Godzik
Sep 1, 1986·Journal of Neurochemistry·R SpectorD Kinzenbaw

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.