PMID: 2509113Oct 1, 1989Paper

A new colorimetric assay for purine nucleoside phosphorylase

Clinical Biochemistry
S Y ChuM Jiang

Abstract

A new colorimetric procedure for the determination of purine nucleoside phosphorylase (PNP, E.C. 2.4.2.1) activity is described. In this procedure, the hydrogen peroxide formed in the PNP-xanthine oxidase reaction is used to oxidize the chromogenic reagents--3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone using the enzyme peroxidase. The rate of enzyme reaction is followed at 520 nm. This procedure correlated well with the UV method (290 nm), with a correlation coefficient of 0.98 (P less than 0.005). Within-run and between-run precision (CV) were less than 2.8% and 3.7%, respectively. Here we also describe an optimized NAD-dependent method (340 nm) for PNP determination. The colorimetric method is superior to both the 340 nm and the UV methods in terms of both sensitivity and precision. The mean erythrocyte PNP activity for 17 healthy subjects was 11.88 U/mL packed cells for the NAD-dependent method and 13.22 U/mL packed cells for the colorimetric method.

References

Jan 1, 1978·Analytical Biochemistry·M P UitendaalC Griscelli
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E R MablyF F Snyder
May 1, 1971·Annals of Human Genetics·Y H EdwardsH Harris
Oct 16, 1980·The New England Journal of Medicine·J BlattD G Poplack

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Citations

May 5, 2001·Pharmacology & Therapeutics·A BzowskaD Shugar
Jan 23, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shelli SchomakerJiri Aubrecht
Feb 18, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Roberto I López-CruzTania Zenteno-Savín
Oct 28, 2015·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Iris Del Castillo Velasco-MartínezTania Zenteno-Savín

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