PMID: 2484438Jun 1, 1989Paper

A new colorimetric nucleic acid hybridization assay for Listeria in foods

International Journal of Food Microbiology
W KingJ D Klinger

Abstract

Non-isotopic colorimetric detection has been applied in a rapid nucleic acid dipstick hybridization assay for detection of Listeria spp. in food and environmental samples. The assay takes approximately 2.5-3 h following a two day broth and plate enrichment. Hybridization occurs between fluorescein labeled detector probes, poly(deoxyadenosine)-tailed capture probes, and Listeria-specific regions of 16 S ribosomal RNA. These target:probe complexes are captured on poly(deoxythymidine) coated plastic dipsticks. Detection is based on binding of horseradish peroxidase conjugated anti-fluorescein antibody to the hybridization complex, and enzyme-mediated color development. The colorimetric endpoint is read on a photometer at 450 nm. In these initial studies, 306 inoculated dairy, meat and seafood samples, and 200 environmental samples were tested. When compared with total culture results the hybridization assay had unconfirmed positive and false-negative rates of approximately 1.4-2.9% and 0.8-4.7%, respectively.

References

Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J LaneN R Pace
Jun 1, 1989·International Journal of Food Microbiology·W KingJ D Klinger
Jun 1, 1989·International Journal of Food Microbiology·J M Farber
Sep 29, 1988·The New England Journal of Medicine·M J LinnanR Weaver
Jan 1, 1988·Infection·H P Seeliger

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Citations

Dec 1, 1996·Molecular Biotechnology·E P Groody
Jun 1, 1989·International Journal of Food Microbiology·W KingJ D Klinger
Nov 1, 1995·International Journal of Food Microbiology·J E OlsenO Olsvik
Jul 1, 1992·The Journal of Applied Bacteriology·S FitterC J Thomas
Oct 1, 1994·FEMS Microbiology Reviews·H HofstraJ van der Plas
Feb 1, 1991·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J E OlsenB B Nielsen
Dec 19, 2020·Comprehensive Reviews in Food Science and Food Safety·Carlo Spanu, Kieran Jordan
Jul 1, 2014·Comprehensive Reviews in Food Science and Food Safety·Vincenzina Fusco, Grazia Marina Quero
May 1, 1999·Applied and Environmental Microbiology·D M Norton, C A Batt
Sep 1, 1993·Applied and Environmental Microbiology·B W Blais, L M Phillippe
Nov 1, 1990·Applied and Environmental Microbiology·E SkjerveO Olsvik
Apr 1, 1991·Clinical Microbiology Reviews·A SchuchatC V Broome
Sep 1, 1991·Microbiological Reviews·J M Farber, P I Peterkin
Oct 1, 1992·Clinical Microbiology Reviews·M J Wolcott
Dec 1, 1991·Applied and Environmental Microbiology·R F WangM G Johnson
Nov 6, 2020·Analytical Chemistry·Rebeca S RodriguezChristy L Haynes

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