A new confirmatory Neisseria gonorrhoeae real-time PCR assay targeting the porA pseudogene

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
D M WhileyT P Sloots

Abstract

The Roche Cobas Amplicor system is widely used for the detection of Neisseria gonorrhoeae but is known to cross react with some commensal Neisseria spp. Therefore, a confirmatory test is required. The most common target for confirmatory tests is the cppB gene of N. gonorrhoeae. However, the cppB gene is also present in other Neisseria spp. and is absent in some N. gonorrhoeae isolates. As a result, laboratories targeting this gene run the risk of obtaining both false-positive and false-negative results. In the study presented here, a newly developed N. gonorrhoeae LightCycler assay (NGpapLC) targeting the N. gonorrhoeae porA pseudogene was tested. The NGpapLC assay was used to test 282 clinical samples, and the results were compared to those obtained using a testing algorithm combining the Cobas Amplicor System (Roche Diagnostics, Sydney, Australia) and an in-house LightCycler assay targeting the cppB gene (cppB-LC). In addition, the specificity of the NGpapLC assay was investigated by testing a broad panel of bacteria including isolates of several Neisseria spp. The NGpapLC assay proved to have comparable clinical sensitivity to the cppB-LC assay. In addition, testing of the bacterial panel showed the NGpapLC assay to be highl...Continue Reading

Citations

Dec 21, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A WalshB Crowley
Jun 14, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Leon P McNallyPhilip H Cunningham
Nov 7, 2007·Journal of Clinical Microbiology·Silke Peter-GetzlaffBurkhard Springer
Feb 18, 2011·Journal of Clinical Microbiology·Annie ChengJames E Kirby
Jan 27, 2006·The Journal of Molecular Diagnostics : JMD·David M WhileyTheo P Sloots
Oct 27, 2006·The Journal of Molecular Diagnostics : JMD·Stig Ove HjelmevollVegard Skogen
May 10, 2005·Diagnostic Microbiology and Infectious Disease·David M WhileyTheo P Sloots
Feb 26, 2014·International Journal of STD & AIDS·Danielle Albuquerque Pires RochaCristina Maria Borborema dos Santos
Mar 26, 2008·Sexual Health·David M WhileyJohn W Tapsall
Jul 6, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Magnus UnemoHans Fredlund
Jan 11, 2017·Nature Reviews. Urology·Magnus Unemo, Jorgen S Jensen
Apr 3, 2010·Journal of Clinical Pathology·Isabelle O'CallaghanBrigid Lucey
Apr 29, 2021·Journal of Medical Microbiology·Amanda BordinDavid M Whiley

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