PMID: 8580907Dec 1, 1995Paper

A new dominant selectable marker for genetic transformation; Hsp70-opd

Insect Biochemistry and Molecular Biology
M Q BenedictF H Collins

Abstract

New P element plasmids containing the organophosphate-degrading gene opd as a dominant selectable marker were tested as transformation vectors in Drosophila melanogaster. One of these vectors was modified by the addition of the D. melanogaster mini-white gene as a comarker. When transformed individuals were identified using paraoxon selection for opd alone, results were similar to those obtained with mini-white. No false positives were recovered, however one strain contained the mini-white gene but inadequate resistance to survive our screening regimen due to a defective Hsp70-opd gene. Results suggest that Hsp70-opd is similar to mini-white for distinguishing transformed individuals but does not require time-consuming individual examination. Due to the mode of action of organophosphorus nerve agents, Hsp70-opd has potential as a selectable marker in numerous animals beside fruit flies.

References

Feb 15, 1990·Archives of Biochemistry and Biophysics·D P DumasJ R Wild
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P PhillipsJ R Wild
Aug 1, 1984·Cell·R E Karess, G M Rubin
Oct 22, 1982·Science·G M Rubin, A C Spradling

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Citations

Mar 1, 1997·Parasitology Today·D A O'Brochta, P W Atkinson
Feb 13, 2001·Insect Biochemistry and Molecular Biology·A M Handler
Sep 13, 2002·Insect Biochemistry and Molecular Biology·Carsten HornErnst A Wimmer
May 22, 2004·Bulletin of Entomological Research·M G Kramer
Dec 29, 1998·Emerging Infectious Diseases·C B BeardF F Richards
Jul 14, 1998·Insect Molecular Biology·M AshburnerJ J Peloquin
Oct 2, 2007·Development·Koen J T Venken, Hugo J Bellen

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