A New Ethylene-Responsive Factor CaPTI1 Gene of Pepper (Capsicum annuum L.) Involved in the Regulation of Defense Response to Phytophthora capsici

Frontiers in Plant Science
Jing-Hao JinZhen-Hui Gong

Abstract

Ethylene-responsive factors (ERF) are usually considered to play diverse roles in plant response to biotic and abiotic stresses. In this study, an ERF gene CaPTI1 was isolated from pepper transcriptome database. CaPTI1 contains an open reading frame (ORF) of 543 bp, which encodes a putative polypeptide of 180 amino acids with a theoretical molecular weight of 20.30 kDa. Results of expression profile showed that CaPTI1 had a highest expression level in roots and this gene could not only response to the infection of Phytophthora capsici and the stresses of cold and drought, but also be induced by the signaling molecule (salicylic acid, Methyl Jasmonate, Ethephon, and hydogen peroxide). Furthermore, virus-induce gene silencing (VIGS) of CaPTI1 in pepper weakened the defense response significantly by reducing the expression of defense related genes CaPR1, CaDEF1 and CaSAR82 and also the root activity. These results suggested that CaPTI1 is involved in the regulation of defense response to P. capsici in pepper.

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Citations

Jun 30, 2018·Genome Génome / Conseil National De Recherches Canada·Jing-Hao JinZhen-Hui Gong
Aug 13, 2020·Molecular Plant-microbe Interactions : MPMI·Solhee InDoil Choi
Mar 3, 2017·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Jianguang LiuHanshuang Zhang
Mar 17, 2020·Frontiers in Plant Science·Huai-Xia ZhangZhen-Hui Gong

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Methods Mentioned

BETA
two-hybrid
transgenic
environmental stresses

Software Mentioned

Statistical Analysis System SAS
MEGA
DNAMAN
ExPASy

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