A new family of RSF1010-derived expression and lac-fusion broad-host-range vectors for gram-negative bacteria

Gene
M LabesR Simon

Abstract

A series of broad-host-range expression and lac fusion vectors, based on RSF1010 derivatives, was constructed. The expression vectors contain various promoters (pNm, plac, ptac and pS1) for expression of foreign genes. The efficiency of the promoters was determined in Escherichia coli, Rhizobium meliloti, Rhizobium leguminosarum and Pseudomonas putida by beta-galactosidase activity measurements. Of the promoters assayed in E. coli, the most effective is the tac promoter, whereas in soil bacteria the appropriate promoter for overexpression of foreign genes is the NmR promoter. The GmR gene, serving as a selectable marker for the plasmids, was efficiently expressed in R. meliloti as revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and thus, pGm was also used to construct an expression vector. The translational fusion vectors allow the identification and characterization of promoter-carrying cloned fragments on the translational level, whereas the transcriptional fusion vectors can be used to identify and to study promoters on cloned fragments. All lac fusion vectors contain the E. coli lacZ gene or the complete lac operon facilitating quantification of expression.

References

Sep 1, 1985·Plasmid·Y D Tsygankov, A Y Chistoserdov
Nov 1, 1986·Plasmid·A Y Chistoserdov, Y D Tsygankov
Jan 1, 1987·Molecular & General Genetics : MGG·K M Derbyshire, N S Willetts
Jan 1, 1986·Gene·I BorosP Venetianer
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·V HaringM Bagdasarian
Sep 1, 1974·Journal of General Microbiology·J E Beringer
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E ScherzingerM Bagdasarian
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski

❮ Previous
Next ❯

Citations

Sep 16, 2003·Journal of Immunological Methods·Myung-Hee KwonHyung-Il Kim
Jun 21, 2012·Molecular Plant-microbe Interactions : MPMI·Fenghuan YangChenyang He
Aug 30, 2007·Molecular Plant-microbe Interactions : MPMI·Susana Castro-SowinskiYaacov Okon
Aug 7, 2009·Molecular Plant-microbe Interactions : MPMI·Ming GuoJames R Alfano
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Francisco Goes da SilvaPamela C Ronald
Jun 16, 2004·Molecular Plant-microbe Interactions : MPMI·Saul BurdmanPamela Ronald
Sep 21, 2010·Journal of Bacteriology·Jonathan GisinBernd Masepohl
Dec 26, 2006·Journal of Bacteriology·Veronika M MeierBirgit E Scharf
Jul 21, 2009·Journal of Bacteriology·Veronika M Meier, Birgit E Scharf
Sep 19, 2006·Journal of Bacteriology·Christine RotterBirgit Scharf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.