A new fluorescent probe for neutral to alkaline pH and imaging application in live cells.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Qingqing MaYuying Liu

Abstract

A new pH-sensitive fluorescent probe NAP-MDA was designed and synthesized. NAP-MDA consists of 1,8-naphthalimide as fluorophore, morpholine and N,N-dimethylethylenediamine as pH-responsive groups. Due to the photoinduced electron transfer (PET) mechanism, the fluorescence of 1, 8-naphthalimide was thoroughly quenched under alkaline condition (pH > 10.0), however, NAP-MDA displayed increasing fluorescence as the rise of acidity. Notably, NAP-MDA possessed an excellent linear dependence with neutral to alkaline pH (7.2-9.4), with a pKa of 8.38. NAP-MDA had good photostability and reversibility. Meanwhile, the probe was selective to pH without interference from common reactive species, temperature and viscosity. Fluorescent testing strips were fabricated with NAP-MDA and were successfully utilized to visualize the different pH with a handhold UV lamp. Confocal fluorescence imaging in live cells demonstrated that NAP-MDA mainly fluoresced in lysosomes, and could be applied for quantification of the pH within live cells.

References

Dec 1, 1984·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·J L Wike-HooleyH S Reinhold
Nov 30, 2000·Critical Care : the Official Journal of the Critical Care Forum·J A Kellum
May 17, 2005·Journal of Biochemical and Biophysical Methods·Miao YuqingFang Keming
Aug 12, 2005·Chemical Reviews·Jacopo TomasiRoberto Cammi
Oct 17, 2009·Chemical Reviews·Junyan Han, Kevin Burgess
Nov 24, 2012·Nature Chemistry·Jefferson ChanChristopher J Chang
Jun 8, 2013·Accounts of Chemical Research·Dan DingBen Zhong Tang
Nov 19, 2013·Journal of the American Chemical Society·Hyung Joong KimHwan Myung Kim
Aug 27, 2014·Journal of the American Chemical Society·Min Hee LeeJong Seung Kim
Oct 7, 2014·Chemical Society Reviews·Min Hee LeeJonathan L Sessler
Jan 23, 2016·Scientific Reports·Jiangli FanXiaojun Peng
Mar 21, 2017·Chemical Society Reviews·Ji-Ting HouJong Seung Kim
Dec 12, 2014·Methods and Applications in Fluorescence·Wen ShiHuimin Ma
Jan 2, 2018·Royal Society Open Science·G S LukaH Najjaran
Apr 25, 2018·Journal of the American Chemical Society·Shodai TakahashiYasuteru Urano
Nov 16, 2018·Chemical Communications : Chem Comm·Min Jung ChangMin Hee Lee
May 21, 2019·Chemical Communications : Chem Comm·Li FanChuan Dong
Jul 25, 2019·Accounts of Chemical Research·Minggang TianWeiying Lin
Sep 21, 2019·Accounts of Chemical Research·Wen SunXiaojun Peng
Nov 22, 2019·Chemical Society Reviews·Sang-Hyun ParkInjae Shin

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