A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos

Development, Growth & Differentiation
Kosho MoritaniKoji Akasaka

Abstract

We report the identification and characterization of Unichrom, a gene encoding a new G-stretch-DNA-binding protein in the sea urchin embryo. The derived amino acid sequence of Unichrom contains plant homeodomain (PHD) finger and high mobility group (HMG) motifs as well as motifs required for cell-cycle-dependent degradation. The expression of a Unichrom-green fluorescent protein (GFP) fusion protein in sea urchin embryonic cells indicates that Unichrom protein accumulates in nuclei during interphase and disperses into the cytoplasm at mitosis. Overexpression of dominant negative Unichrom, which contains the DNA binding domain lacking the motif for cell-cycle-dependent degradation, causes impairment of chromosome segregation. These results suggest that Unichrom binds to genome DNA at G-stretch and that degradation of Unichrom is required for segregation of chromosomes.

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Citations

Sep 21, 2011·Journal of Biochemistry·Haruna TakagiNaoaki Sakamoto
May 6, 2008·PLoS Genetics·Kazuma HanaiSusumu Hirose
Mar 21, 2006·Zoological Science·Hideki TagashiraKoji Akasaka
Oct 22, 2013·Gene Expression Patterns : GEP·Yiannis DrososVassiliki Aleporou-Marinou
Aug 23, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoshi WatanabeKoji Akasaka

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