A new gas chromatographic method for the estimation of reserpine and rescinnamine

Journal of Chromatography
G SettimjM R Del Giudice


Under the conditions described for alkaline hydrolysis of reserpine and rescinnamine in absolute and aqueous methanol, and after esterification (with diazomethane) of the resulting acid fraction, methyl 3,4,5-trimethoxybenzoate was quantitatively recovered, whereas methyl trans-3,4,5-trimethoxycinnamate, in normal lighting conditions, was either partly isomerized to methyl cis-trimethoxycinnamate or formed an adduct with a molecule of methanol, yielding methyl 3-methoxy-3-(3,4,5-trimethoxyphenyl)propionate. The structures of the products were established by synthesis, nuclear magnetic resonance studies and mass spectrometry. This investigation of the hydrolytic conditions allowed a reliable and rapid gas chromatographic determination of reserpine and/or rescinnamine in amounts down to 500 and 2000 mug, respectively, to be devised.


Apr 1, 1966·Journal of Gerontology·H P von Hahn


Jun 9, 2007·Analytical and Bioanalytical Chemistry·Javier López-FloresAntonio Molina-Díaz
Feb 21, 2002·Phytochemistry·Renata Vasques da SilvaM Furlan

Related Concepts

Chromatography, Gas-Liquid
Hydrogen-Ion Concentration
Study, Methodological
In Vivo NMR Spectroscopy
Mass Spectrometry

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