A new genotype of nephropathogenic infectious bronchitis virus circulating in vaccinated and non-vaccinated flocks in China

Avian Pathology : Journal of the W.V.P.A
Shengwang Liu, Xiangang Kong

Abstract

Five strains of infectious bronchitis virus (IBV) were isolated from five layer flocks that had nephropathogenic infection in four provinces in China. Among them, three of the five flocks had been vaccinated against infectious bronchitis. Virulence studies indicated that the five Chinese IBV isolates caused 10 to 30% mortality in 15-day-old specific pathogen free chickens and gross lesions were mainly confined to the kidneys in all of the dead chickens. Two oligonucleotide pairs, S1Uni2 and S1Oligo3' or S1Oligo5' and S1Oligo3', were used after propagation of the isolates in embryonated eggs to amplify the S1 protein genes of the spike protein. The cDNA derived by reverse transcriptase-polymerase chain reaction was cloned and sequenced. The nucleotide and amino acid sequence of S1 protein gene had a similar degree of identity (> or =92%) among the five Chinese IBV isolates. The nucleotide and amino acid identity of the S1 protein gene between the five Chinese IBV isolates and 16 strains of other IBVs varied from 60 to 81%. This clearly showed that the five Chinese IBV isolates comprised a separate genotype. These results demonstrated, for the first time, that there is a new genotype of nephropathogenic IBV circulating in vaccina...Continue Reading

References

Mar 1, 1989·Virology·J G KustersB A van der Zeijst
Jan 1, 1987·The Journal of General Virology·M E BoursnellM M Binns
May 17, 2008·Avian Pathology : Journal of the W.V.P.A·A AdzharD Cavanagh
Sep 1, 1996·Avian Pathology : Journal of the W.V.P.A·K ShawD Cavanagh
Sep 1, 1992·Avian Pathology : Journal of the W.V.P.A·D CavanaghJ K Cook
Apr 1, 2000·Avian Pathology : Journal of the W.V.P.A·J J De Wit

❮ Previous
Next ❯

Citations

Jun 25, 2010·Archives of Virology·Karim MardaniGlenn F Browning
Mar 16, 2006·Avian Pathology : Journal of the W.V.P.A·Dave Cavanagh
Apr 6, 2006·Avian Pathology : Journal of the W.V.P.A·Roser DolzNatàlia Majó
Apr 6, 2006·Avian Pathology : Journal of the W.V.P.A·Richard E GoughDave Cavanagh
Sep 23, 2006·Avian Pathology : Journal of the W.V.P.A·Yury A BochkovVladimir V Drygin
Apr 19, 2011·Avian Pathology : Journal of the W.V.P.A·Jorge Luis ChaconAntonio J Piantino Ferreira
Apr 9, 2010·Viral Immunology·Dan YuShengwang Liu
Nov 10, 2015·Avian Pathology : Journal of the W.V.P.A·Qianqian XuShengwang Liu

❮ Previous
Next ❯

Datasets Mentioned

BETA
A24863
KB8523
M21515
X87238
L18990
L10384
X15832
M21970
AF352315
AF274438

Methods Mentioned

BETA
electron microscopy
PCR

Software Mentioned

DNAMAN

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.