A new GLT1 splice variant: cloning and immunolocalization of GLT1c in the mammalian retina and brain

Neurochemistry International
Thomas RauenDavid V Pow

Abstract

We have identified a novel carboxyl-terminal splice-variant of the glutamate transporter GLT1, which we denote as GLT1c. Within the rat brain only low levels of protein and message were detected, protein expression being restricted to end feet of astrocytes apposed to blood vessels or some astrocytes adjacent to the ventricles. Conversely, within the retina, this variant was selectively and heavily expressed in the synaptic terminals of both rod- and cone-photoreceptors in both humans and rats. Double-immunolabelling with antibodies to the carboxyl region of GLT1b/GLT1v, which is strongly expressed in apical dendrites of bipolar cells and in cone photoreceptors revealed that in the rat GLT1c was co-localised with GLT1b/GLT1v in cone photoreceptors but not with GLT1b/GLT1v in bipolar cells. GLT1c expression was developmentally regulated, only appearing at around postnatal day 7 in the rat retina, when photoreceptors first exhibit a dark current. Since the glutamate transporter EAAT5 is also expressed in terminals of rod photoreceptor terminals these data indicate that rod photoreceptors express two glutamate transporters with distinct properties. Similarly, cone photoreceptors express two glutamate transporters. We suggest that ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jan 1, 1984·Journal of Neurochemistry·F Fonnum
Dec 1, 1996·Cell and Tissue Research·T RauenH Wässle
Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·J L ArrizaS G Amara
Dec 31, 1997·FEBS Letters·N Utsunomiya-TateY Kanai
Mar 17, 1999·The Journal of Clinical Investigation·A S Fanning, J M Anderson
Oct 26, 1999·Progress in Retinal and Eye Research·W B Thoreson, P Witkovsky
May 23, 2001·Progress in Neurobiology·N C Danbolt
Oct 3, 2002·Journal of Neurochemistry·Carol L Poitry-YamateThomas Rauen
Feb 6, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Weizhi ChenPaul A Rosenberg

❮ Previous
Next ❯

Citations

Nov 19, 2013·Pflügers Archiv : European journal of physiology·Christof GrewerThomas Rauen
Mar 3, 2009·Molecular Neurobiology·George Zanazzi, Gary Matthews
Dec 21, 2010·Asian Journal of Andrology·Aven LeeDavid V Pow
Aug 7, 2007·Cytogenetic and Genome Research·S KatyalR Godbout
Nov 25, 2011·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·James S MeabonDavid G Cook
Aug 4, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yuchuan LiNiels Christian Danbolt
Sep 3, 2010·The Journal of Physiology·Matthew J M RowanWen Shen
Dec 14, 2011·Progress in Retinal and Eye Research·Mohammadali AlmasiehAdriana Di Polo
Oct 5, 2010·The International Journal of Biochemistry & Cell Biology·Aven Lee, David V Pow
May 4, 2010·Experimental Eye Research·David J HolcombeNigel L Barnett
Jun 2, 2009·Journal of Chemical Neuroanatomy·Yasuro Atoji, Mohammad Rafiqul Islam
Dec 31, 2008·Neurochemistry International·Andreas BringmannAndreas Reichenbach
May 23, 2007·Neurochemistry International·Amanda L Sheldon, Michael B Robinson
Jul 28, 2005·Journal of Neurochemistry·Marco I GonzálezMichael B Robinson
Nov 1, 2008·The European Journal of Neuroscience·Rebecca G HuMichael Kalloniatis
Aug 23, 2008·Glia·Marcello MeloneFiorenzo Conti
Jan 11, 2008·The European Journal of Neuroscience·Merav BassanPaul A Rosenberg
Oct 7, 2004·Brain Research. Developmental Brain Research·David V PowPaul B Colditz
Feb 5, 2015·Journal of Neuroscience Research·Mari-Carmen MarxBrian Billups
Oct 2, 2014·Journal of Neurochemistry·Georgi Gegelashvili, Ole J Bjerrum
Jun 3, 2015·Cellular and Molecular Life Sciences : CMLS·Kou TakahashiChien-Liang Glenn Lin
Jun 10, 2015·Translational Psychiatry·S M O'DonovanR E McCullumsmith
May 29, 2016·Neurochemistry International·N C DanboltY Zhou
Dec 21, 2006·Neuroreport·Lauren T MacnabDavid V Pow

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Barrel cortex

Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.