A new hepatoma cell line for toxicity testing at repeated doses

Cell Biology and Toxicology
Nicolas FabreJoan-Albert Vericat

Abstract

Many cell models that are used to assess basic cytotoxicity show a good correlation with acute toxicity. However, their correlation with the toxicity seen following chronic in vivo exposure is less evident. The new human hepatoma cell line HBG BC2 possesses the capacity of being reversibly differentiated in vitro and of maintaining a relatively higher metabolic rate when in the differentiated state (3 weeks) as compared to HepG2 cells, and thus may allow the conduct of repeated toxicity testing on cells in culture. In order to evaluate the genetic background of HBG BC2 cells, the expression of selected genes was analyzed in untreated cultures and, in addition, the behavior of HBG BC2 cultures under conditions of repeated treatment was studied with acetaminophen as a test substance and coupled with the use of standard staining techniques to demonstrate toxicity. Results showed that cultures of HBG BC2 cells retained a capacity to undergo apoptosis and proliferation, allowing probable replacement of damaged cells in the culture monolayer. MTT reduction was used to evaluate the toxicity of acetaminophen, acetylsalicylic acid, perhexiline, and propranolol, after both single and repeated (3 times/week for 2 weeks) administration. Un...Continue Reading

Citations

Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Moo-Yeal LeeDouglas S Clark
Jun 17, 2008·Cell Biology and Toxicology·G Dicky John DavisR Jayakumar
Apr 25, 2012·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Alexandra BurkardJoris Braspenning
Mar 9, 2006·Alternatives to Laboratory Animals : ATLA·Sandra CoeckeAndrew Worth
Jul 27, 2006·Expert Opinion on Drug Metabolism & Toxicology·José V CastellMaría José Gómez-Lechón
May 8, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Helga Tuschl, Christina E Schwab
Jul 17, 2007·Journal of Pharmaceutical Sciences·Ana Ruiz-GarciaVicente G Casabo

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