A new heterofunctional support for enzyme immobilization: PEI functionalized Fe3 O4 MNPs activated with divinyl sulfone. Application in the immobilization of lipase from Thermomyces lanuginosus

Enzyme and Microbial Technology
Rayanne M BezerraJosé C S Dos Santos

Abstract

Lipase from Thermomyces lanuginosus (TLL) was immobilized onto a novel heterofunctional support, divinyl sulfone (DVS) superparamagnetic nanoparticles (SPMNs) functionalized with polyethyleneimine (PEI). Particle size and zeta potential measurements, elemental analysis, X-ray powder diffraction, magnetic measurements, and infrared spectroscopy analysis were used to characterize the TLL preparations. At pH 10, it was possible to achieve 100 % of immobilization yield in 1 h. The immobilization pH gives TLL preparations with different stabilities; indeed the TLL preparation immobilized at pH 5.0 was the most stable during the thermal inactivation at all pH values. For the hydrolysis of racemic methyl mandelate, the nanobiocatalysts immobilized at pH 5.0 and blocked with ethylenediamine (EDA) and ethanolamine (ETA) obtained good enantioselectivities (68 % and 72 %, respectively) with high catalytic activities in the reaction medium at pH 7.0. The operational stability of the systems was evaluated in the esterification reaction of benzyl alcohol, obtaining up to 61 % conversion after the seventh reaction cycle. These results show that SPMN@PEI-DVS support is a robust strategy for the easy and rapid recovery of the nanobiocatalyst by...Continue Reading

Citations

Jul 28, 2020·Critical Reviews in Food Science and Nutrition·Asghar Taheri-KafraniRajender S Varma
Nov 25, 2020·International Journal of Biological Macromolecules·Zahra AshkanAmin Tashakor
Nov 5, 2020·International Journal of Biological Macromolecules·Muhammad Bilal, Hafiz M N Iqbal
Jan 17, 2021·International Journal of Biological Macromolecules·Soudabeh FarhadiMojtaba Mortazavi
Nov 18, 2020·International Journal of Biological Macromolecules·Mayur R LadoleAniruddha B Pandit
Apr 18, 2021·International Journal of Biological Macromolecules·Yale Luck NunesJosé C S Dos Santos
Jun 15, 2021·International Journal of Biological Macromolecules·Bruna Coelho de AndradeClaucia Fernanda Volken de Souza

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