A new high-performance liquid chromatographic method for the determination and distribution of linalool in Michelia alba.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
En-Qin XiaHua-Bin Li

Abstract

A new high-performance liquid chromatographic method with photodiode array detection was established for the determination of linalool in the plant Michelia alba. Linalool was extracted from the plant sample with the aid of ultrasound, and was analyzed on a Waters RP C(18) column (4.6 x 150 mm, 5 microm) using an acetonitrile and water (55:45, v/v) mobile phase at a flow rate of 1.0 mL/min. The column temperature was set at 25 degrees C, and the detection wavelength was 210 nm. The linear range of the method was 5-200 microg/mL with a correlation coefficient of 0.9975. The recovery was 92-112%, and the relative standard deviation was 1.85% (n = 9). The present method has been used to study the distribution of linalool in the plant Michelia alba. The plant samples include flowers, leaves and tender twigs. Furthermore, leaves included samples in their tender, grown-up and fallen phases, and flowers included samples in their juvenile, middle and whitening phases. The concentrations of linalool in different parts of the plant were 0.21-0.65%, 1.63-4.89% and 0.43% for leaves, flowers and tender twigs, respectively. The results showed that all the plant materials contained relative high concentration of linalool, and juvenile phase f...Continue Reading

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Citations

Dec 4, 2012·International Journal of Environmental Research and Public Health·Yong-Hong ChenHua-Bin Li
Dec 4, 2012·International Journal of Environmental Research and Public Health·Yong-Hong ChenHua-Bin Li
Dec 22, 2012·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Dinesh KumarOm Prakash
Dec 4, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yu-Jie ZhangHua-Bin Li
Jun 15, 2014·Journal of Ethnopharmacology·Chanai NoysangThomas Efferth
Aug 10, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Veena PandeyShyamal K Nandi

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