A New High Throughput Screening Platform for Cell Encapsulation in Alginate Hydrogel Shows Improved Hepatocyte Functions by Mesenchymal Stromal Cells Co-encapsulation.

Frontiers in Medicine
Valeria IansanteCeline Filippi

Abstract

Hepatocyte transplantation has emerged as an alternative to liver transplant for liver disease. Hepatocytes encapsulated in alginate microbeads have been proposed for the treatment of acute liver failure, as they are able to provide hepatic functions while the liver regenerates. Furthermore, they do not require immunosuppression, as the alginate protects the hepatocytes from the recipient's immune cells. Mesenchymal stromal cells are very attractive candidates for regenerative medicine, being able to differentiate into cells of the mesenchymal lineages and having extensive proliferative ability. When co-cultured with hepatocytes in two-dimensional cultures, they exert a trophic role, drastically improving hepatocytes survival and functions. In this study we aimed to (i) devise a high throughput system (HTS) to allow testing of a variety of different parameters for cell encapsulation and (ii) using this HTS, investigate whether mesenchymal stromal cells could have beneficial effects on the hepatocytes when co-encapsulated in alginate microbeads. Using our HTS platform, we observed some improvement of hepatocyte behavior with MSCs, subsequently confirmed in the low throughput analysis of cell function in alginate microbeads. Ther...Continue Reading

References

Jan 1, 1996·Plastic and Reconstructive Surgery·K T PaigeC A Vacanti
Jan 29, 2000·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·B M BilirJ Durham
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Koichi MasudaEugene J-M A Thonar
Apr 16, 2003·Cell Transplantation·Ragai R MitryAnil Dhawan
Jul 2, 2003·Biomaterials·Hyun Joon KongDavid J Mooney
Jun 10, 2006·Journal of Inherited Metabolic Disease·Anil DhawanRobin D Hughes
Apr 26, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Serafim M OliveiraMário A Barbosa
Oct 1, 2008·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Anil Dhawan
Jan 28, 2009·Nature Reviews. Immunology·Antonio UccelliVito Pistoia
Jan 28, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Claire TerryRobin D Hughes
Apr 7, 2010·Nature Reviews. Gastroenterology & Hepatology·Anil DhawanRagai R Mitry
Jun 10, 2010·The Journal of Biological Chemistry·Koichi WatashiKuan-Teh Jeang
Oct 30, 2010·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Ricky Harminder BhogalSimon C Afford
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
Apr 26, 2012·Nature Reviews. Immunology·Katarina Le Blanc, Dimitrios Mougiakakos
Jun 27, 2012·Tissue Engineering. Part a·Alejandro Gómez-Aristizábal, John Edward Davies
Nov 28, 2013·Carbohydrate Polymers·Therese AndersenBjørn E Christensen
Dec 19, 2013·Biomacromolecules·Keila B FonsecaCristina C Barrias
Jul 19, 2014·Mitochondrion·L B BuravkovaB Zhivotovsky
May 20, 2015·Immunology Letters·Katarina Le Blanc, Lindsay C Davies
Oct 22, 2016·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Volker M LauschkeMagnus Ingelman-Sundberg
Jan 1, 2015·Microarrays·Therese AndersenMichael Dornish
Nov 11, 2016·Methods in Molecular Biology·Raphael P H MeierLeo H Bühler
Nov 11, 2016·Methods in Molecular Biology·Ragai R MitryAnil Dhawan
Mar 3, 2017·Journal of Cellular Biochemistry·Dalia Abdelrahim Alzebdeh, Howard William Matthew
Mar 26, 2013·Materials·Jinchen Sun, Huaping Tan
Sep 14, 2017·Cell Transplantation·Suttiruk JitraruchRagai R Mitry
Dec 16, 2017·Scientific Reports·Heba BadraiqDusko Ilic
Jan 23, 2018·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Charlotte A LeeEmer Fitzpatrick

❮ Previous
Next ❯

Citations

Dec 5, 2019·Tissue Engineering. Part B, Reviews·Elnaz Sadat MirdamadiMehran Solati-Hashjin
Feb 27, 2020·Expert Review of Gastroenterology & Hepatology·Minh Phuong NguyenAnil Dhawan
Jan 12, 2021·Molecular and Cellular Probes·Rebecca Marie DewhurstJohn A Sayer
Apr 13, 2020·Methods : a Companion to Methods in Enzymology·Steven Vermeulen, Jan de Boer
Jun 15, 2021·Acta Biomaterialia·Da ZouChun-Xia Zhao
Jul 3, 2021·Chemical Reviews·Farhad SoheilmoghaddamJustin Cooper-White

❮ Previous
Next ❯

Methods Mentioned

BETA
Protein Assay
Enzyme Linked Immunosorbent
ELISA
Assay

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.