A new highly discriminatory multiplex capillary-based MLVA assay as a tool for the epidemiological survey of Pseudomonas aeruginosa in cystic fibrosis patients.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
D SobralChristine Pourcel

Abstract

Multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) has been shown to provide a high level of information for epidemiological investigations and the follow-up of Pseudomonas aeruginosa chronic infection. In the present study, an automatized MLVA assay has been developed for the analysis of 16 VNTRs in two multiplex polymerase chain reactions (PCRs), followed by capillary electrophoresis. The result in the form of a code is directly usable for clustering analyses. This MLVA-16(Orsay) scheme was applied to the genotyping of 83 isolates from eight cystic fibrosis patients, demonstrating that the same genotype persisted during eight years of chronic infection in the majority of cases. Comparison with pulsed-field gel electrophoresis (PFGE) analysis showed that both methods were congruent, MLVA providing, in some cases, additional informativity. The evolution of strains during long-term infection was revealed by the presence of VNTR variants.

References

Jan 1, 1987·The Journal of Hospital Infection·D P Speert, M E Campbell
Dec 1, 1994·The Journal of Infectious Diseases·U RömlingB Tümmler
Nov 1, 1996·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H Vu-ThienA Garbarg-Chenon
Nov 8, 2003·Journal of Clinical Microbiology·Lucie OntenienteGilles Vergnaud
Jan 17, 2004·Clinical Microbiology Reviews·Lisa Saiman, Jane Siegel
Dec 8, 2004·Journal of Clinical Microbiology·Barry CurranChristopher G Dowson
Feb 3, 2005·JAMA : the Journal of the American Medical Association·Zhanhai LiMark L Splaingard
Apr 26, 2007·Protein Science : a Publication of the Protein Society·Richard C WatersKieran A Ryan
May 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Lutz WiehlmannBurkhard Tümmler
Sep 21, 2007·Journal of Clinical Microbiology·Jennifer K JohnsonAnthony D Harris
Jun 13, 2009·Methods in Molecular Biology·Gilles Vergnaud, Christine Pourcel
Sep 8, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J F TurtonT L Pitt
Apr 9, 2010·Clinical Microbiology Reviews·John J Lipuma

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Citations

May 1, 2013·The Journal of Antimicrobial Chemotherapy·Catherine LlanesUNKNOWN GERPA Study Group
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Dong-Sheng Lian, Shu-Jin Zhao
May 13, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yolande HauckChristine Pourcel
Dec 3, 2014·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·E JohanssonA Lindblad
Sep 4, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Milena Danda Vasconcelos SantosMaria Betânia Melo de Oliveira
May 11, 2021·FEMS Microbiology Reviews·Andreu Coello PelegrinAlex van Belkum

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