A new highly sensitive point of care screen for spontaneous bacterial peritonitis using the leukocyte esterase method

Journal of Hepatology
Michel Henry MendlerBruce A Runyon

Abstract

Urine reagent strips measuring leukocyte esterase activity have been studied to screen spontaneous bacterial peritonitis (SBP) but are insensitive. We calibrated a strip specifically for ascitic fluid to achieve high sensitivity in this diagnosis. Experiments were conducted on ascitic fluid from patients with cirrhosis. Samples with SBP were diluted with native acellular ascitic fluid to achieve PMN counts below, above, and close to the diagnostic threshold of 250 PMN/microl. A model of SBP was created by spiking negative ascitic fluid samples (<250 PMN/microl) with activated PMN from blood of patients with sepsis, and diluted to achieve a range of PMN. Aliquots were tested at 2, 3, 4, and 10 min with the Periscreen leukocyte esterase strip. PMN/microl was correlated to timings and color scales: white defined negative (PMN <250/microl); and shades of brown, purple, and pink defined positive. Ascitic fluid samples were obtained from 58 patients. Negative ascitic fluid was used from 32 to generate the model SBP. One thousand three hundred and four experiments were performed with a median PMN count of 492/microl (0-7510). After exclusion of uninterpretable colorimetric results, 1089 experiments were analyzed [PMN of 444/microl (0-...Continue Reading

References

Feb 1, 1987·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·D KutterL Klemmer
Jul 6, 1996·BMJ : British Medical Journal·J H BeerP Cottagnoud
Jan 12, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Javier FernándezJuan Rodés
Jun 20, 2002·Artificial Organs·Ramin SamVasant C Gandhi
Nov 20, 2002·European Journal of Gastroenterology & Hepatology·Geoffroy VanbiervlietAlbert Tran
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·José CastelloteXavier Xiol
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Luke T EvansPatrick S Kamath
Mar 5, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Bruce A Runyon, UNKNOWN Practice Guidelines Committee, American Association for the Study of Liver Diseases (AASLD)
May 29, 2004·European Journal of Gastroenterology & Hepatology·Thierry ThévenotNoureddine Merzoug
Feb 3, 2005·Journal of Gastroenterology and Hepatology·Thierry SapeyMichel-Henry Mendler
Mar 11, 2005·Journal of Clinical Gastroenterology·Jose CastelloteXavier Xiol
Jul 19, 2005·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·P W BarnesUNKNOWN international consensus group for hematology
Dec 14, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Do Young KimYoung Myoung Moon
Nov 8, 2006·Clinical Chemistry and Laboratory Medicine : CCLM·Robert de JongeJan Lindemans
Nov 23, 2006·São Paulo Medical Journal = Revista Paulista De Medicina·Lucia Libanez Bessa Campelo BragaAntônio Haroldo de Araújo Filho
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jean-Baptiste NousbaumUNKNOWN Association Nationale des Hépato-Gastroentérologues des Hôpitaux Généraux de France
Oct 9, 2007·The American Journal of Emergency Medicine·Brian Chinnock, Gregory W Hendey
Oct 31, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Veit GülbergBeate Appenrodt
Mar 13, 2008·JAMA : the Journal of the American Medical Association·Camilla L WongSharon E Straus
Oct 7, 2008·World Journal of Gastroenterology : WJG·Oliviero RiggioManuela Merli

❮ Previous
Next ❯

Citations

Mar 31, 2011·World Journal of Gastroenterology : WJG·Anastasios Koulaouzidis
Sep 12, 2013·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Stefano FagiuoliPierluigi Toniutto
Aug 19, 2014·International Journal of Hepatology·Hirayuki EnomotoShuhei Nishiguchi
Mar 15, 2011·Clinical Gastroenterology and Hepatology : the Official Clinical Practice Journal of the American Gastroenterological Association·Alexander R BonnelK Rajender Reddy
Sep 12, 2015·La Presse médicale·Jean-Baptiste Nousbaum
Mar 27, 2015·International Journal of Critical Illness and Injury Science·Kiran ChughPradeep Kumar
Jul 2, 2016·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Hideto KawarataniHitoshi Yoshiji
Sep 14, 2016·The American Journal of Gastroenterology·Thierry ThévenotUNKNOWN CFEHTP, ANGH and the PerDRISLA study group
Apr 8, 2015·World Journal of Hepatology·Philipp LutzUlrich Spengler
May 5, 2020·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Xianmei WuTao Luo
Jul 28, 2020·Scandinavian Journal of Gastroenterology·Maryam NasereslamiZahra Jalali
Mar 11, 2016·World Journal of Hepatology·Chalermrat BunchorntavakulDisaya Chavalitdhamrong
Jun 17, 2020·European Journal of Gastroenterology & Hepatology·Alexandros HadjivasilisPanayiotis Kouis
Jan 2, 2018·The American Journal of Emergency Medicine·Brit Long, Alex Koyfman

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.