PMID: 7029530Aug 1, 1981Paper

A new hypothalamic substance, and not luteinizing hormone-releasing hormone, is detected immunocytochemically by antibody to luteinizing hormone-releasing hormone

Proceedings of the National Academy of Sciences of the United States of America
L A SternbergerW G Forssmann

Abstract

Adjacent paraffin sections of rat hypothalami fixed in Bouin's fluid were treated either with buffer or with luteinizing hormone-releasing hormone (LHRH) before immunocytochemical staining with anti-LHRH. Upon buffer pretreatment, pituitary gonadotrophs were unstained and hypothalamic fibers were stained. Upon LHRH pretreatment, pituitary gonadotrophs were stained (receptor reaction) and hypothalamic fibers were unstained. Extension of washes and use of series of neutralizing antisera between LHRH application and immunocytochemical staining, as well as the absence of inhibiting concentrations of LHRH in the later washes and neutralizing antisera removed from the sections, excluded the possibility that the disappearance of visualization of hypothalamic fibers was due to blockage of anti-LHRH in immunocytochemical staining. The results suggested that LHRH removed from the sections an immunocytochemically stainable but as yet unknown analog of LHRH and replaced it with LHRH, which in turn became lost during subsequent immunocytochemical processing. This idea was confirmed by the isolation by high-pressure liquid chromatography of a peak, distinct from LHRH, upon treatment of hypothalami with LHRH. It is suggested that the new subs...Continue Reading

References

Jan 1, 1978·General and Comparative Endocrinology·B Scharrer
Sep 17, 1975·Cell and Tissue Research·L A Sternberger, J P Petrali
Nov 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S A Joseph, L A Sternberger
Jul 27, 1979·Science·D T Krieger, A S Liotta
Sep 1, 1977·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L A Sternberger, J P Petrali
Dec 28, 1978·Histochemistry·D GrubeE Weber
Dec 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C TougardS Avrameas
Dec 1, 1979·Neuroscience Letters·R MartinK H Voigt
Jul 1, 1979·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·M Nozaki, H Kobayashi
May 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L I LarssonE Solcia
Apr 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·C H Li, D Chung
Jan 1, 1980·Cell and Tissue Research·H A FritschA G Pearse

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Citations

Oct 1, 1984·Journal of the Neurological Sciences·J A BigginsJ A Edwardson
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L A SternbergerN H Sternberger
Jan 1, 1983·Annals of the New York Academy of Sciences·N H Sternberger, L A Sternberger

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