A new immunodeficient retinal dystrophic rat model for transplantation studies using human-derived cells

Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie
Biju ThomasMark S Humayun

Abstract

To create new immunodeficient Royal College of Surgeons (RCS) rats by introducing the defective MerTK gene into athymic nude rats. Female homozygous RCS (RCS-p+/RCS-p+) and male nude rats (Hsd:RH-Foxn1mu, mutation in the foxn1 gene; no T cells) were crossed to produce heterozygous F1 progeny. Double homozygous F2 progeny obtained by crossing the F1 heterozygotes was identified phenotypically (hair loss) and genotypically (RCS-p+ gene determined by PCR). Retinal degenerative status was confirmed by optical coherence tomography (OCT) imaging, electroretinography (ERG), optokinetic (OKN) testing, superior colliculus (SC) electrophysiology, and by histology. The effect of xenografts was assessed by transplantation of human embryonic stem cell-derived retinal pigment epithelium (hESC-RPE) and human-induced pluripotent stem cell-derived RPE (iPS-RPE) into the eye. Morphological analysis was conducted based on hematoxylin and eosin (H&E) and immunostaining. Age-matched pigmented athymic nude rats were used as control. Approximately 6% of the F2 pups (11/172) were homozygous for RCS-p+ gene and Foxn1mu gene. Homozygous males crossed with heterozygous females resulted in 50% homozygous progeny for experimentation. OCT imaging demonstrat...Continue Reading

References

Sep 15, 1978·Science·A I Goldman, P J O'Brien
Feb 1, 1991·Clinical Biochemistry·J A ThliverisK R Copeland
Aug 1, 1966·The Journal of Comparative Neurology·R SiminoffL Kruger
Jan 1, 1997·Experimental Animals·T HirasawaS Makino
Mar 4, 2000·Human Molecular Genetics·P M D'CruzD Vollrath
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·D VollrathM M LaVail
Feb 21, 2002·Japanese Journal of Ophthalmology·Salvatore GrisantiKlaus Heimann
Jul 26, 2002·Experimental Eye Research·Robert B Aramant, Magdalene J Seiler
Mar 27, 2003·Investigative Ophthalmology & Visual Science·Botir T SagdullaevMaureen A McCall
Jun 9, 2004·Experimental Eye Research·Biju B ThomasRobert B Aramant
Aug 25, 2004·Journal of Neuroscience Methods·Biju B ThomasRobert B Aramant
Nov 10, 2004·Eye·H A Quigley, D S Iglesia
Jun 14, 2005·Neuroscience Letters·Biju B ThomasMagdalene J Seiler
Nov 22, 2005·Transplantation Proceedings·D CibulskyteJ Mortensen
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Deepak A LambaThomas A Reh
Oct 3, 2006·Cloning and Stem Cells·Raymond D LundRobert Lanza
Jan 4, 2008·Investigative Ophthalmology & Visual Science·Shaomei WangRaymond D Lund
Jun 13, 2008·American Journal of Ophthalmology·Norman D RadtkeMagdalene J Seiler
Jun 27, 2008·Investigative Ophthalmology & Visual Science·Shaomei WangRaymond D Lund
Feb 4, 2010·Cell and Tissue Research·Aditi RayEun-Jin Lee
Mar 9, 2010·Biochemical and Biophysical Research Communications·Fengming YueKatsunori Sasaki
Mar 20, 2010·Advances in Experimental Medicine and Biology·Emeline F Nandrot, Eric M Dufour
Jan 27, 2012·The European Journal of Neuroscience·Trevor J McGillAlexandra Capela
May 18, 2012·The European Journal of Neuroscience·Laura ClarkeDerek van der Kooy
Jul 10, 2012·Progress in Retinal and Eye Research·Magdalene J Seiler, Robert B Aramant
Jul 17, 2012·Neuron·Jayakrishna Ambati, Benjamin J Fowler
Nov 13, 2012·Cell and Tissue Research·Colleen L ZhuNorberto M Grzywacz
May 13, 2014·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Magdalene J SeilerHans S Keirstead

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.