A New Indirect Spectrofluorimetric Method for Determination of Ascorbic Acid with 2,4,6-Tripyridyl-S-Triazine in Pharmaceutical Samples

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Lejla KlepoLea Kukoc-Modun

Abstract

Ascorbic acid (AA) is a water-soluble vitamin which shows no fluorescence. However, in reaction with iron(III), AA is oxidised to dehydroascorbic acid and iron(III) is reduced to iron(II) which forms a complex with 2,4,6-tripyridyl-S-triazine (TPTZ) in buffered medium. The relative fluorescence intensity of the resulting Fe(TPTZ)₂(2+) complex can be measured at excitation and emission wavelengths of 393 and 790 nm, respectively. Based on this data, a new indirect spectrofluorimetric method for the determination of AA in pharmaceutical samples was proposed. Influence of the reaction conditions, such as acidity of acetic buffer, concentration of TPTZ and iron(III), reaction time and instrumental parameters were investigated in detail. The linear range was from 5.4 × 10(-4) to 5.4 × 10(-6) mol·L(-1) (R = 0.9971). The LOD was 7.7 × 10(-7) mol·L(-1) and LOQ was 2.3 × 10(-4) mol·L(-1). Fourteen pharmaceutical samples containing various amounts of AA were analysed. Influences of potential interfering substances were also examined. Analysis of commercial pharmaceutical formulations showed good correlation with the nominal values given by the manufacturers and with the results obtained by a titration method. The proposed method can be a...Continue Reading

References

Nov 16, 2001·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Y FujitaM Shimano
Jun 14, 2002·Journal of Pharmaceutical and Biomedical Analysis·Isabel AlberoConcepción Sánchez-Pedreño
May 7, 2004·Journal of Pharmaceutical and Biomedical Analysis·Tomás Pérez-RuizAna Guillén
Jul 27, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lun WangFeng Gao
Oct 6, 2005·Chemical & Pharmaceutical Bulletin·Yusuf Dilgin, Gürel Nişli
Dec 21, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Behzad RezaeiSiavash Nouroozi
Jun 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Abdolkarim AbbaspourAbolhassan Noori

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