A new kind of aptamer-based immunomagnetic electrochemiluminescence assay for quantitative detection of protein

Biosensors & Bioelectronics
Debin ZhuDa Xing

Abstract

A new kind of aptamer-based immunomagnetic electrochemiluminescence (IM-ECL) assay for quantitative detection of protein is developed. The assay consists of a double aptamer sandwich format in which a biotin-labeled aptamer is used for rapid and specific capture of target protein, and a [Ru(bpy)(3)](2+) (TBR)-labeled aptamer is used for ECL detection. As an example, platelet-derived growth factor B-chain homodimer (PDGF-BB) was detected by the method. Experimental results show that the detection limit of the assay is 80 pmol/L of PDGF-BB. A calibration curve with a linearity range from 0.1 to 1000 nmol/L is established, thus, make quantitative analysis possible. The method has been used to detect PDGF-BB in fetal calf serum with minimum background interference. Due to the wide availability of aptamer for numerous proteins, this new method holds great promise in protein detection.

Citations

Apr 26, 2014·Microchemical Journal, Devoted to the Application of Microtechniques in All Branches of Science·John G BrunoTaylor Phillips
Apr 22, 2015·Biosensors & Bioelectronics·Fenglei GaoDaoquan Tang
Feb 24, 2017·Biosensors & Bioelectronics·Kateryna MuzykaGuobao Xu
Jul 10, 2020·Analytical and Bioanalytical Chemistry·Xinyue KouLimin Ning

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