A new l-amino acid oxidase from Bothrops jararacussu snake venom: Isolation, partial characterization, and assessment of pro-apoptotic and antiprotozoal activities

International Journal of Biological Macromolecules
Sante E I CaroneSuely V Sampaio

Abstract

A new l-amino acid oxidase (LAAO) from Bothrops jararacussu venom (BjussuLAAO-II) was isolated by using a three-step chromatographic procedure based on molecular exclusion, hydrophobicity, and affinity. BjussuLAAO-II is an acidic enzyme with pI=3.9 and molecular mass=60.36kDa that represents 0.3% of the venom proteins and exhibits high enzymatic activity (4884.53U/mg/mim). We determined part of the primary sequence of BjussuLAAO-II by identifying 96 amino acids, from which 34 compose the N-terminal of the enzyme (ADDRNPLEECFRETDYEEFLEIARNGLSDTDNPK). Multiple alignment of the partial BjussuLAAO-II sequence with LAAOs deposited in the NCBI database revealed high similarity (95-97%) with other LAAOs isolated from Bothrops snake venoms. BjussuLAAO-II exerted a strong antiprotozoal effect against Leishmania amazonensis (IC50=4.56μg/mL) and Trypanosoma cruzi (IC50=4.85μg/mL). This toxin also induced cytotoxicity (IC50=1.80μg/mL) and apoptosis in MCF7 cells (a human breast adenocarcinoma cell line) by activating the intrinsic and extrinsic apoptosis pathways, but were not cytotoxic towards MCF10A cells (a non-tumorigenic human breast epithelial cell line). The results reported herein add important knowledge to the field of Toxinology,...Continue Reading

Citations

Apr 17, 2018·PLoS Neglected Tropical Diseases·Stephanie StranskyVania Maria Martin Braga
Aug 7, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Patrícia Heloise Alves BezerraMaria Regina Torqueti
May 28, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ana Rita Thomazela MachadoLusânia Maria Greggi Antunes
Nov 27, 2020·International Journal of Biological Macromolecules·Luana Gonçalves BarbosaRenata Santos Rodrigues
May 20, 2020·Seminars in Cancer Biology·Félix A Urra, Ramiro Araya-Maturana
Nov 24, 2019·International Journal of Biological Macromolecules·Thiago Geraldo SoaresMárcia Helena Borges
Jun 10, 2021·Applied Microbiology and Biotechnology·Kosuke KasaiTomisato Miura
Jun 20, 2020·Toxicon : Official Journal of the International Society on Toxinology·Jacqueline Ramos Machado BragaMarisa Maria Teixeira da Rocha

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