A new lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for rapid detection of tetrodotoxin

The Analyst
Haicong ShenYong Tang

Abstract

Tetrodotoxin (TTX) is a potent, low molecular weight analyte that can lead to fatal poisoning and requires a sensitive, rapid detection method. Here, we have developed a competitive, lateral-flow immunochromatographic strip combined with quantum dot nanobeads (QDNBs) and gold nanoflowers (AuNFs). This approach is called turn-on C-LFICS and it meets all testing requirements. Subsequent analysis revealed that this turn-on C-LFICS was rapid (8 min), sensitive (LOD = 0.2 ng mL-1), and quantitative (DLR = 1.56-100 ng mL-1), and had a positive signal readout (based on fluorescence quenching effects) for TTX detection. Moreover, it had superior signal brightness and a low background interference signal when compared with previous methods. Finally, it can function free of interference from the sample matrix and has a demonstrated recovery range of 85.5% to 119.7% in spiked samples. Taken together, these results show that our turn-on C-LFICS is an effective detection tool for TTX or other small molecules.

References

Sep 29, 2004·Toxicon : Official Journal of the International Society on Toxinology·Margaret A O'LearyGeoffrey K Isbister
Mar 3, 2005·Journal of the American Chemical Society·C S YunG F Strouse
Jun 2, 2005·Nature Materials·Igor L MedintzHedi Mattoussi
Feb 2, 2006·Analytical Chemistry·Joung-Hwan ChoSe-Hwan Paek
Aug 10, 2007·Biomaterials·Timothy JamiesonAlexander M Seifalian
Sep 4, 2007·Trends in Biochemical Sciences·David W Piston, Gert-Jan Kremers
Feb 12, 2009·ACS Nano·Jianping XieDaniel I C Wang
Jun 23, 2010·Journal of the American Chemical Society·Ying WangYuehe Lin
Jan 5, 2011·Journal of Agricultural and Food Chemistry·Betsy Jean YakesStacey L Degrasse
Dec 14, 2011·Marine Drugs·Kelvin Sze-Yin LeungYeuk-Ki Tsoi
Jan 30, 2013·Biosensors & Bioelectronics·Kobra OmidfarMaedeh Darziani Azizi
Feb 26, 2014·Toxins·Vaishali BaneAmbrose Furey
Mar 16, 2016·Chemical Communications : Chem Comm·Wen RenJoseph Irudayaraj
Mar 19, 2016·Biosensors & Bioelectronics·Huihua QuYan Zhao

❮ Previous
Next ❯

Citations

Sep 15, 2018·International Journal of Molecular Sciences·Joshua M CampbellAdam T Melvin
Jan 5, 2021·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Poorvisha Ravi, Muthupandian Ganesan
Mar 17, 2020·Ecotoxicology and Environmental Safety·Yifeng LanChuan Dong
Jan 15, 2022·Journal of Food Science·Ruibin XieAiliang Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
surface
enzyme-linked immunosorbent assay
FRET
ELISA
i-ELISA
Fluorescence

Software Mentioned

fluorescence ICS reader

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.