A new Leishmania-specific hypothetical protein and its non-described specific B cell conformational epitope applied in the serodiagnosis of canine visceral leishmaniasis

Parasitology Research
Daniela P LageEduardo Antonio Ferraz Coelho

Abstract

The serodiagnosis of canine visceral leishmaniasis (CVL) presents problems related to its sensitivity and/or specificity. In the present study, a new Leishmania-specific hypothetical protein, LiHyD, was produced as a recombinant protein (rLiHyD) and evaluated in ELISA experiments for the CVL serodiagnosis. LiHyD was characterized as antigenic in a recent immunoproteomic search performed with Leishmania infantum proteins and the sera of dogs developing visceral leishmaniasis (VL). Aiming to compare the efficacy between whole proteins and synthetic peptides, two linear and one conformational B cell epitopes of LiHyD were synthesized and also evaluated as diagnostic markers. The four antigens were recognized by the sera of dogs suffering VL. On the contrary, low reactivity was observed when they were assayed with sera from non-infected healthy dogs living in endemic or non-endemic areas of leishmaniasis. In addition, no reactivity was found against them using sera from dogs experimentally infected by Trypanosoma cruzi, Babesia canis, or Ehrlichia canis, or sera from animals vaccinated with the Leish-Tec® vaccine, a prophylactic preparation commercially available for CVL prevention in Brazil. As comparative diagnostic tools, a reco...Continue Reading

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Citations

Nov 29, 2016·Journal of Biomolecular Structure & Dynamics·Fauzia JamalShubhankar K Singh
Dec 21, 2018·PloS One·Ana Maria Ravena Severino CarvalhoDaniel Menezes-Souza
Jul 7, 2019·Tropical Medicine & International Health : TM & IH·Isis Polianna Silva Ferreira de CarvalhoMaria Regina Fernandes de Oliveira
May 22, 2019·Acta Parasitologica·Mehdi NamavariMohammad Asadpour
Jan 13, 2018·PLoS Neglected Tropical Diseases·Bruno L TraviGuadalupe Miró
Mar 17, 2019·Parasitology Research·Vanessa da Silva RibeiroJulia Maria Costa-Cruz
Aug 16, 2019·Research in Veterinary Science·Juliana M MachadoEduardo A F Coelho
Aug 23, 2018·Trends in Parasitology·Malcolm S DuthieOrin Courtenay

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