A new maltose-inducible high-performance heterologous expression system in Bacillus subtilis

Biotechnology Letters
Jie YueJianping Wen

Abstract

To improve heterologous proteins production, we constructed a maltose-inducible expression system in Bacillus subtilis. An expression system based on the promoter for maltose utilization constructed in B. subtilis. Successively, to improve the performance of the P malA -derived system, mutagenesis was employed by gradually shortening the length of P malA promoter and altering the spacing between the predicted MalR binding site and the -35 region. Furthermore, deletion of the maltose utilization genes (malL and yvdK) improved the P malA promoter activity. Finally, using this efficient maltose-inducible expression system, we enhanced the production of luciferase and D-aminoacylase, compared with the P hpaII system. A maltose-inducible expression system was constructed and evaluated. It could be used for high level expression of heterologous proteins production.

References

Aug 10, 2005·Applied Microbiology and Biotechnology·Igor Mierau, Michiel Kleerebezem
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen
May 19, 2006·Journal of Bacteriology·Stefan SchönertMichael K Dahl
Jul 14, 2010·Microbial Cell Factories·Yang M MingGong Y Sheng
Nov 1, 2012·Microbial Cell Factories·Anna A ToymentsevaThorsten Mascher
Jun 3, 2014·Microbial Cell Factories·Huina Dong, Dawei Zhang
Jul 6, 2014·Journal of Microbiology and Biotechnology·Haifeng ZhangDawei Zhang
Jan 28, 2016·Molecular Microbiology·Jara RadeckGeorg Fritz

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