A new marker for mosaic analysis in Caenorhabditis elegans indicates a fusion between hyp6 and hyp7, two major components of the hypodermis.

Genetics
J YochemM Han

Abstract

A fusion of the sur-5 protein to the green fluorescent protein containing a nuclear localization signal is demonstrated as a marker for genetic mosaic analysis in the nematode Caenorhabditis elegans. Because of an extensive accumulation of bright fluorescence in many nuclei, normal growth plates, each containing hundreds of worms, can be rapidly screened with a dissecting microscope for rare mosaic individuals. As the marker can also be used to detect transgenic worms, the construction of strains for mosaic analyses can be minimized. In the course of examining rare mosaic animals, an unexpected pattern of fluorescence was noticed for hyp6, a syncytial component of the hypodermis, which indicated that the marker may serve as a means of assessing cellular fusions during development. Immunofluorescent staining of adherens junctions confirmed a postembryonic fusion of hyp6 with hyp7, the major syncytium of the hypodermis.

References

Mar 1, 1977·Developmental Biology·J E Sulston, H R Horvitz
May 1, 1974·Genetics·S Brenner
Nov 1, 1983·Developmental Biology·J E SulstonJ N Thomson
Feb 23, 1995·Nature·R HeimR Y Tsien
Feb 11, 1994·Science·M ChalfieD C Prasher
Feb 1, 1994·Developmental Biology·B Podbilewicz, J G White
Nov 1, 1995·Genetics·E M Hedgecock, R K Herman

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Citations

Aug 30, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Takafumi KonishiAsako Sugimoto
Aug 8, 2007·Gene·Hiroshi QadotaKozo Kaibuchi
Mar 15, 2005·Molecular Genetics and Genomics : MGG·John YochemRobert K Herman
Jan 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Beth E ThompsonJudith Kimble
Nov 7, 2006·Developmental Cell·Mara Schvarzstein, Andrew M Spence
Nov 23, 2006·Developmental Biology·James D McGheeA Gordon Robertson
May 4, 2005·Developmental Cell·Kenji KontaniJoel H Rothman
Oct 14, 1998·Trends in Genetics : TIG·J Hodgkin, R K Herman
Dec 16, 2006·Molecular Genetics and Genomics : MGG·Ryuji Minasaki, Adrian Streit
Mar 21, 2006·Developmental Biology·Brian L Nelms, Wendy Hanna-Rose
Mar 28, 2006·Developmental Cell·Veerle RottiersAdam Antebi
Sep 30, 2004·Journal of Cell Science·Hiroki MoribeEisuke Mekada
Oct 7, 2004·Current Opinion in Neurobiology·Christopher T Zugates, Tzumin Lee
Oct 28, 2004·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yukako AoyamaHiroshi Shibuya
Aug 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christian E RocheleauMeera V Sundaram
Jun 9, 2006·BMC Genetics·Aidyl S Gonzalez-Serricchio, Paul W Sternberg
Nov 24, 2004·Current Biology : CB·Yukihiko KubotaKiyoji Nishiwaki

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