PMID: 9529076Apr 7, 1998Paper

A new member of the S-layer protein family: characterization of the crs gene from Campylobacter rectus

Infection and Immunity
B WangD Kolodrubetz

Abstract

Strains of the periodontal pathogen Campylobacter rectus express a 150- to 166-kDa protein on their cell surface. This protein forms a paracrystalline lattice, called the surface layer (S-layer), on the outer membrane of this gram-negative bacterium. To initiate a genetic analysis of the function of the S-layer in the pathogenesis of C. rectus, we have cloned and characterized its gene. The S-layer gene (crs) from C. rectus 314 encodes a cell surface protein which does not have a cleaved signal peptide at its amino terminus. Although the amino acid sequence deduced from the crs gene has 50% identity with the amino-terminal 30 amino acids of the four S-layer proteins from Campylobacter fetus, the similarity decreases to less than 16% over the rest of the protein. Thus, the crs gene from C. rectus encodes a novel S-layer protein whose precise role in pathogenesis may differ from that of S-layer proteins from other organisms. Southern and Northern blot analyses with probes from different segments of the crs gene indicate that the S-layer gene is a single-copy, monocistronic gene in C. rectus. RNA end mapping and sequence analyses were used to define the crs promoter; there is an exact match to the Escherichia coli -10 promoter con...Continue Reading

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Citations

Jun 10, 2010·FEMS Microbiology Reviews·Irena LinhartováPeter Sebo
Jan 29, 2000·Journal of Bacteriology·M Sára, U B Sleytr
Feb 28, 2002·Oral Microbiology and Immunology·D HinodeR Nakamura
Jan 17, 2012·Protoplasma·Victoria G LewisChristopher A McDevitt
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Jul 15, 2005·Annals of Periodontology·Stuart A Thompson
Dec 16, 1998·Journal of Bacteriology·S A ThompsonM J Blaser
Jul 2, 2003·FEMS Immunology and Medical Microbiology·Heinz P Hahn, Bernd Ulrich von Specht
Oct 20, 2005·Journal of Bioscience and Bioengineering·Kenji Omori, Akiko Idei

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