A new method allowing long-term potentiation recordings in hippocampal organotypic slices

Brain and Behavior
Paula PaciLaurence Ris

Abstract

Hippocampal organotypic slices are used to improve the understanding of synaptic plasticity mechanisms because they allow longer term studies compared to acute slices. However, it is more delicate to keep cultures alive in the recording system outside in vitro conditions. Experiments from the organotypic cultures are common but the handling of slices is rarely described in the literature, even though tissue preservation is crucial. Instruments are sometimes required to extract the slices from the culture inserts but this approach is delicate and can lead to damage, given how strongly the slices are attached to the insert. A new configuration is proposed to secure the transfer of slices from the incubator to the recording chamber through an adaptor piece that can be designed for any model of chamber and/or insert. The adaptor is a Plexiglas ring in which a culture insert containing the slice can be easily introduced and stabilized. This system allows slices to be placed in the interface for electrophysiological investigations without having to detach them from the insert. That way, no damage is caused and the recording system can safely hold the slices, maintaining them close to culture conditions. In addition to the description...Continue Reading

References

Mar 6, 1992·Brain Research·L E Chavez-Noriega, C F Stevens
Apr 1, 1991·Journal of Neuroscience Methods·L StoppiniD Muller
Dec 1, 1981·Journal of Neuroscience Methods·B H Gähwiler
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y Y Huang, E R Kandel
Jan 7, 1993·Nature·T V Bliss, G L Collingridge
Jan 15, 1993·Brain Research. Developmental Brain Research·P A BuchsD Muller
Jan 15, 1993·Brain Research. Developmental Brain Research·D MullerL Stoppini
Feb 6, 1997·Nature·U Frey, R G Morris
Mar 1, 1997·Journal of Neurophysiology·C CollinM Segal
Jul 10, 2003·Trends in Neurosciences·Haruo KasaiHiroyuki Nakahara
Sep 10, 2003·Neural Plasticity·Ken ShimonoGary Lynch
Oct 28, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marta ZagrebelskyMartin Korte
Feb 20, 2007·Journal of Neuroscience Methods·William W Anderson, Graham L Collingridge
May 16, 2009·Journal of Neuroscience Methods·Komal RambaniSteve M Potter
Feb 2, 2012·Journal of Neuroinflammation·Jonathan VinetKnut Biber
Jun 17, 2014·Scientific Reports·Yossi BuskilaJohn W Morley

❮ Previous
Next ❯

Citations

Feb 8, 2019·Scientific Reports·César R Romero-LeguizamónKristi A Kohlmeier

❮ Previous
Next ❯

Methods Mentioned

BETA
dissection
transfection
light
Fluorescence
confocal microscopy
fluorescence microscopy

Software Mentioned

‐ ASW
FV10
Filament tracer
Filament Tracer Imaris
TILLvisION
TILL vis ION
R
FV
Excel
WinLTP

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.