PMID: 2499351Mar 21, 1989Paper

A new method for determining the heat capacity change for protein folding

Biochemistry
C N Pace, D V Laurents

Abstract

In order to use results from calorimetry or thermal unfolding curves to estimate the free energy change for protein unfolding at 25 degrees C, it is necessary to know the change in heat capacity for unfolding, delta Cp. We describe a new method for measuring delta Cp which is based on results from urea and thermal unfolding curves but does not require a calorimeter. We find that delta Cp = 1650 +/- 200 cal/(deg.mol) for the unfolding of ribonuclease T1 and that delta Cp = 2200 +/- 300 cal/(deg.mol) for the unfolding of ribonuclease A.

References

Jan 1, 1976·Biophysical Chemistry·W Pfeil, P L Privalov
Dec 1, 1987·Analytical Biochemistry·C N PaceB J Barnett
Jan 1, 1988·Annual Review of Biophysics and Biophysical Chemistry·D P Goldenberg
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·Y V Griko Venyaminov SYu
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R L Baldwin
Nov 1, 1987·Biopolymers·W J Becktel, J A Schellman
Aug 5, 1986·Journal of Molecular Biology·P L PrivalovV P Kutyshenko
Jul 5, 1974·Journal of Molecular Biology·P L Privalov, N N Khechinashvili
May 1, 1974·Biophysical Chemistry·E I Tiktopulo, P L Privalov

❮ Previous
Next ❯

Citations

Mar 1, 1992·Biopolymers·J Edelman
Jul 1, 1992·Protein Science : a Publication of the Protein Society·D A Schultz, R L Baldwin
Dec 1, 1993·Protein Science : a Publication of the Protein Society·L Swint, A D Robertson
Jan 1, 1995·Protein Science : a Publication of the Protein Society·J M Scholtz
Mar 1, 1995·Protein Science : a Publication of the Protein Society·Y Kawata, K Hamaguchi
Jul 1, 1996·Protein Science : a Publication of the Protein Society·C G GenzorJ Sancho
Jan 1, 1993·Progress in Biophysics and Molecular Biology·M Oobatake, T Ooi
Feb 1, 1996·International Journal of Biological Macromolecules·C PomboM N Jones
Aug 7, 2013·International Journal of Biological Macromolecules·Irene Sánchez MoránEnrique Villar
Oct 15, 2003·Journal of Molecular Biology·Ariel A Di NardoAlan R Davidson
Apr 15, 2004·Phytochemistry·Alexandre Donizeti Martins CavagisHiroshi Aoyama
Jul 18, 1997·Biochimica Et Biophysica Acta·A Weingand-ZiadéP Masson
May 30, 2002·Biophysical Chemistry·Dieter PerlFranz X Schmid
Sep 20, 2012·Journal of the American Chemical Society·Robert D GrayJonathan B Chaires
Dec 6, 2001·European Journal of Biochemistry·M MasipJ G Gavilanes
Dec 20, 2000·European Journal of Biochemistry·U Arnold, R Ulbrich-Hofmann
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L C AntoninoA L Fink
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·T Ooi, M Oobatake
Oct 20, 2011·Protein Engineering, Design & Selection : PEDS·Mar Rodríguez-RodríguezM Angeles Jiménez
Jan 16, 2002·Protein Science : a Publication of the Protein Society·Srebrenka RobicSusan Marqusee
Apr 21, 1999·Protein Science : a Publication of the Protein Society·J K Kaushik, R Bhat
Dec 14, 1999·Protein Science : a Publication of the Protein Society·A ChakrabartiR Varadarajan
Jul 28, 1999·Protein Science : a Publication of the Protein Society·C N PaceG I Makhatadze
Nov 16, 2012·PloS One·Rachel E HelblingMichele Cascella
Feb 7, 2014·Physical Chemistry Chemical Physics : PCCP·Indrani JhaPannuru Venkatesu
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Matthew Auton, D Wayne Bolen
Sep 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Srebrenka RobicSusan Marqusee
Jun 1, 1995·Protein Science : a Publication of the Protein Society·R KhuranaJ B Udgaonkar
Nov 7, 2009·European Biophysics Journal : EBJ·Sanne M NabuursCarlo P M van Mierlo
Jan 1, 1990·Trends in Biochemical Sciences·C N Pace
Oct 24, 2012·FEBS Letters·Elena V EremeevaWillem J H van Berkel
Jul 15, 1995·European Journal of Biochemistry·P SpuerginG E Schulz
Jul 3, 2013·Protein Science : a Publication of the Protein Society·Alexander Tischer, Matthew Auton
Oct 26, 2010·Journal of Bioscience and Bioengineering·Harmen H J de JonghStef J Koppelman
Jul 6, 2010·Biochimica Et Biophysica Acta·Md Zahid KamalNalam Madhusudhana Rao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.