A new method for identifying the amino acid attached to a particular RNA in the cell

FEBS Letters
T SuzukiK Watanabe

Abstract

To investigate the function of tRNAs or any other aminoacylable RNAs in vivo, it is important to be able to estimate the amounts and species of aminoacylated RNAs in living cells. We have developed a method of analyzing amino acids attached to particular tRNAs obtained from cells. After the ester bond between the amino acid and the 3'-adenosine moiety of a specific aminoacyl-tRNA is stabilized by acetylation of the amino acid with [14C]acetic anhydride, the aminoacyl-tRNA can be fished out with a solid-phase-attached DNA probe. The 14C-labeled acetylamino acid is then released from the thus purified acetyl-aminoacyl-tRNAs by alkaline treatment and detected by TLC analysis.

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Citations

Sep 1, 1997·Nucleic Acids Research·I HayashiK Watanabe
Aug 15, 1998·Biological Chemistry·I NittaK Watanabe
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Asuteka NagaoTsutomu Suzuki
Jan 14, 2017·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Yoshiaki Kamada
Oct 29, 2017·Nature Communications·Takaaki AkaikeHozumi Motohashi
Jul 30, 2021·PLoS Genetics·Ziwei LiuMark A Kay

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