A new method for isolating host-independent variants of Bdellovibrio bacteriovorus using E. coli auxotrophs

The Open Microbiology Journal
A Dashiff, Daniel Kadouri

Abstract

Bdellovibrios are Gram-negative bacteria that are characterized by predatory behavior. Although Bdellovibrios exhibit an obligatory parasitic life cycle, it is possible to isolate Bdellovibrio variants that no longer require host cells for their growth. In this study, a new method for isolating Bdellovibrio bacteriovorus host-independent (HI) variants was developed. Filtered B. bacteriovorus prey cells were cultured with E. coli diaminopimelic acid (DAP) auxotrophs as host cells. Thereafter, the lysate was plated on DAP minus media, allowing only HI colonies to develop. Using this method, we have isolated numerous HI variants and demonstrated that the emergence of HI variants may be occurring at a higher frequency than was previously suggested.

References

Aug 21, 2001·Archives of Microbiology·G Barel, Edouard Jurkevitch
Aug 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Chad W Saltikov, Dianne K Newman
Apr 10, 2007·Journal of Bacteriology·Katy J EvansRenee Elizabeth Sockett
Apr 16, 2008·Journal of Microbiological Methods·Megan A FergusonEileen M Spain
Jul 16, 2008·Applied and Environmental Microbiology·John J TudorAdam Zwolak

Related Concepts

Diaminopimelic Acid
Escherichia coli
Gram-Negative Bacteria
Parasites
Bdellovibrio bacteriovorus
Laboratory Culture
Host Defense
SHMT2 gene
Host Cell
Colony (Cells or Organisms)

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