A new method for seed oil body purification and examination of oil body integrity following germination

Journal of Biochemistry
J T TzenJ M Chiu

Abstract

Plant seeds store triacylglycerols as energy sources for germination and postgerminative growth of seedlings. The triacylglycerols are preserved in small, discrete, intracellular organelles called oil bodies. A new method was developed to purify seed oil bodies. The method included extraction, flotation by centrifugation, detergent washing, ionic elution, treatment with a chaotropic agent, and integrity testing by use of hexane. These processes subsequently removed non-specifically associated or trapped proteins within the oil bodies. Oil bodies purified by this method maintained their integrity and displayed electrostatic repulsion and steric hindrance on their surface. Compared with the previous procedure, this method allowed higher purification of oil bodies, as demonstrated by SDS-PAGE using five species of oilseeds. Oil bodies purified from sesame were further analyzed by two-dimensional gel electrophoresis and revealed two potential oleosin isoforms. The integrity of oil bodies in germinating sesame seedlings was examined by hexane extraction. Our results indicated that consumption of triacylglycerols reduced gradually the total amount of oil bodies in seedlings, whereas no alteration was observed in the integrity of rema...Continue Reading

Citations

Mar 28, 2013·Journal of Chemical Biology·Samuel FurseDavid A Gray
Nov 26, 1998·Progress in Lipid Research·J E ThompsonY Hong
Mar 30, 2001·Biochimica Et Biophysica Acta·F BeissonV Arondel
Aug 25, 2010·Journal of Agricultural and Food Chemistry·Bingcan ChenEric Andrew Decker
Jun 23, 2012·Journal of Agricultural and Food Chemistry·Francisca AcevedoThierry Chardot
Sep 12, 2013·Journal of Agricultural and Food Chemistry·Ming-Tsung ChangJason T C Tzen
Mar 2, 2010·Biological & Pharmaceutical Bulletin·Takashi L Shimada, Ikuko Hara-Nishimura
Sep 14, 2010·Bioscience, Biotechnology, and Biochemistry·Hsi-Chi LuJason T C Tzen
Nov 27, 2002·Bioscience, Biotechnology, and Biochemistry·Sorgan S K TaiJason T C Tzen
Jun 24, 2014·Plant Physiology and Biochemistry : PPB·Buntora PasaribuJason T C Tzen
Feb 22, 2016·Plant Physiology and Biochemistry : PPB·Buntora PasaribuJason T C Tzen
Jan 5, 2011·Plant Physiology and Biochemistry : PPB·Georgios BanilasPolydefkis Hatzopoulos
Nov 3, 2010·Plant Physiology and Biochemistry : PPB·Eric S L Hsiao, Jason T C Tzen
Jul 29, 2009·Plant Physiology and Biochemistry : PPB·Pei-Luen JiangJason T C Tzen
Apr 25, 2008·Plant Physiology and Biochemistry : PPB·Anna C N ChuaJason T C Tzen
Oct 19, 2007·Chemosphere·J BoucherI W Marison
Aug 16, 2015·Protein Expression and Purification·Shanyong YiChao Jiang
Jun 30, 2009·Proteomics·Pascale JolivetNathalie Nesi
May 20, 2008·The Plant Journal : for Cell and Molecular Biology·Takashi L ShimadaIkuko Hara-Nishimura
Mar 16, 2012·Journal of Food Science·Ian D Fisk, David A Gray
Nov 3, 2010·Mass Spectrometry Reviews·Ganesh Kumar AgrawalRandeep Rakwal
Sep 1, 2012·Journal of the Science of Food and Agriculture·Ming-Tsung ChangJason T C Tzen
May 26, 2010·The Plant Journal : for Cell and Molecular Biology·María Coca, Blanca San Segundo
Mar 16, 2012·Journal of Food Science·Virginie N KapchiePatricia A Murphy
Jul 6, 2014·Journal of Food Science·Yeming ChenYufei Hua
Aug 28, 2004·Plant Physiology and Biochemistry : PPB·Li-Jen Lin, Jason T C Tzen
Jul 13, 2004·Plant Physiology and Biochemistry : PPB·Pascale JolivetThierry Chardot
Aug 22, 2006·Protein Expression and Purification·Chung-Jen ChiangJason T C Tzen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.