PMID: 265521Feb 1, 1977

A new method for sequencing DNA

Proceedings of the National Academy of Sciences of the United States of America
A M Maxam, W Gilbert

Abstract

DNA can be sequenced by a chemical procedure that breaks a terminally labeled DNA molecule partially at each repetition of a base. The lengths of the labeled fragments then identify the positions of that base. We describe reactions that cleave DNA preferentially at guanines, at adenines, at cytosines and thymines equally, and at cytosines alone. When the products of these four reactions are resolved by size, by electrophoresis on a polyacrylamide gel, the DNA sequence can be read from the pattern of radioactive bands. The technique will permit sequencing of at least 100 bases from the point of labeling.

References

May 4, 1999·Mass Spectrometry Reviews·K D RothJ T Watson
Feb 24, 2001·Electrophoresis·A Guttman, Z Rónai
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Citations

Mar 25, 1975·Biochemistry·J R Lillehaug, K Kleppe
Mar 25, 1976·Journal of Molecular Biology·R J RobertsK Murray
Dec 4, 1973·Biochemistry·J H van de SandeH G Khorana
Jan 1, 1967·Journal of the Chemical Society. Perkin Transactions 1·D H Hayes, F Hayes-Baron
Sep 11, 1964·Biochimica Et Biophysica Acta·E KRIEK, P EMMELOT

Related Concepts

Guanine
Polyacrylamide Gels
Cytosine
Hydrazines
Biochemical Phenomena
Sequencing
Genomic Hybridization
Thymine
DNA Restriction Enzymes
Fragments

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