A new method for simultaneous immunodetection and morphologic identification of individual sources of pollen allergens

The Journal of Allergy and Clinical Immunology
V RazmovskiE R Tovey

Abstract

Exposure to outdoor allergens has commonly been estimated by collecting airborne particles with a Hirst-type spore trap and then using morphologic criteria to identify the intact pollen grains and fungal spores that are recognized as allergen sources. Several antibody-based blotting or fixation methods have also been developed that enable the counting of amorphous airborne particles carrying allergen, but none of these methods allow the ready association of the released allergen with the morphologically identifiable particle of origin. A method has been developed that uses pressure-sensitive adhesive tape to sample the airborne particles and then allows the immunoidentification of the specific particles that are the allergen sources. Our purpose was to visualize and immunostain the particles carrying pollen allergen that are collected with a volumetric spore trap. A Burkard sampler was used to collect airborne particles onto pressure-sensitive adhesive tapes. The particles were permanently fixed between the tape and a protein-binding membrane when the tape was laminated with the membrane. Allergens that elute from the particles onto the membrane were detected with a range of antibodies. Both the particle and associated immunost...Continue Reading

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Citations

Jul 19, 2012·Journal of Exposure Science & Environmental Epidemiology·Félix E Rivera-MarianiPatrick N Breysse
Feb 22, 2012·Journal of Immunological Methods·William R RittenourBrett J Green
Nov 30, 2011·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Jason K SercombeEuan R Tovey
Jul 16, 2003·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·N J CustisT A E Platts-Mills
Sep 4, 2010·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·D Fernández-GonzálezP Mandrioli
Jul 5, 2005·The Science of the Total Environment·K WittmaackR Agerer
Nov 1, 2006·Allergology International : Official Journal of the Japanese Society of Allergology·Brett James GreenEuan Roger Tovey
May 14, 2003·The Journal of Allergy and Clinical Immunology·Monika GroteRudolf Reichelt
Apr 14, 2021·Environmental Research·Chiara SuannoStefano Del Duca
Oct 10, 2002·The Journal of Allergy and Clinical Immunology·Harriet A Burge

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