A new method for the large-scale purification of wheat germ DNA-dependent RNA polymerase II.

Biochemistry
J Jendrisak, R R Burgess

Abstract

An improved method for the purification of the alpha-amanitin-sensitive deoxyribonucleic acid dependent ribonucleic acid polymerase [ribonucleosidetriphosphate:RNA-nucleotidyltransferase, EC 2.7.7.6-A1 (RNA polymerase II or RNA polymerase B) from wheat germ is presented. The method involves homogenization of wheat germ in a buffer of moderate ionic strength, precipitation of RNA polymerase with Polymin P (a polyethylenimine), elution of RNA polymerase from the Polymin P precipitate, ammonium sulfate precipitation, and chromatography on DEAE-cellulose and phosphocellulose. RNA polymerase II is purified over 4000-fold with a 60% recovery, resulting in a yield of 25-30 mg of RNA polymerase from 1 kg of starting material.

Citations

Mar 1, 1987·Plant Molecular Biology·S AckermanE A Davis
Apr 1, 1994·Plant Molecular Biology·P Schweizer, E Mösinger
Jan 1, 1980·Molecular & General Genetics : MGG·S L SeidmanS J Surzycki
Dec 13, 2006·Journal of Molecular Evolution·Jie Luo, Benjamin D Hall
Dec 2, 1977·Biochimica Et Biophysica Acta·E KokkoO Jänne
Mar 28, 1979·Biochimica Et Biophysica Acta·M I McCoyR I Gumport
Dec 14, 1984·Biochemical and Biophysical Research Communications·E Bateman, B H Nicholson
Jan 1, 1981·The International Journal of Biochemistry·Z KilianskaL S Hnilica
Mar 1, 1983·Immunology Letters·D W HoughP J Maddison
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Freiburg
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·D G Blair
Jun 6, 2000·Advanced Drug Delivery Reviews·E Chornet, S Dumitriu
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S RozenblattA Rich
Jan 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·G M WeinstockI R Lehman
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J W Pike, M R Haussler
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K YamazakiN H Chua
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·A KöppenW Wackernagel
Jun 1, 1992·The Journal of Cell Biology·J R HamaguchiE M Bradbury
May 11, 1983·Nucleic Acids Research·D K Trask, M T Muller
Apr 11, 1984·Nucleic Acids Research·D JobM Teissere
Jan 25, 1985·Nucleic Acids Research·P WolffD Riesner
Sep 25, 1979·Nucleic Acids Research·D M LilleyM Houghton
Nov 25, 1981·Nucleic Acids Research·D W Chandler, J Gralla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.