PMID: 6992056Feb 1, 1980Paper

A new method for the partial purification of leishmania amastigotes from cutaneous lesionS

Parasitology
R B InfanteK Dawidowicz

Abstract

Amastigotes from Leishmania braziliensis and L. mexicana were isolated from cutaneous lesions in infected animals using the plant lectin Concanavalin A as a specific agglutination agent. Amastigotes were collected in preparations of up to 95% purity as determined by cell count. The parasites obtained by this method showed no apparent loss of viability as measured by growth rated and DNA replication, or pathogenicity as measured by routine passages in hamsters or mice.

References

Apr 16, 1971·Biochemical and Biophysical Research Communications·M LaurentM Steinert
Sep 1, 1970·The American Journal of Tropical Medicine and Hygiene·H J Akiyama, J C Taylor
Nov 1, 1972·The American Journal of Tropical Medicine and Hygiene·H J Akiyama, N K McQuillen
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Feb 1, 1965·Experimental Parasitology·C L GREENBLATT, P GLASER
Jun 1, 1962·Journal of Molecular Biology·C L SCHILDKRAUTP DOTY

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