A new method for the quantification of monosaccharides, uronic acids and oligosaccharides in partially hydrolyzed xylans by HPAEC-UV/VIS

Carbohydrate Polymers
Dominic LorenzBodo Saake

Abstract

A new method for the chemical characterization of xylans is presented, to overcome the difficulties in quantification of 4-O-methyl-α-D-glucuronic acid (meGlcA). In this regard, the hydrolysis behavior of xylans from beech and birch wood was investigated to obtain the optimum conditions for hydrolysis, using sulfuric acid. Due to varying linkage strengths and degradation, no general method for complete hydrolysis can be designed. Therefore, partial hydrolysis was applied, yielding monosaccharides and small meGlcA containing oligosaccharides. For a new method by HPAEC-UV/VIS, these samples were reductively aminated by 2-aminobenzoic acid. By quantification of monosaccharides and oligosaccharides, as well as comparison with borate-HPAEC and (13)C NMR-spectroscopy, we revealed that the concentrations meGlcA are significantly underestimated compared to conventional methods. The detected concentrations are 85.4% (beech) and 76.3% (birch) higher with the new procedure. Furthermore, the quantified concentrations of xylose were 9.3% (beech) and 6.5% (birch) higher by considering the unhydrolyzed oligosaccharides as well.

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Citations

Aug 10, 2017·Environmental Technology·Friedrich SteffenBodo Saake
May 10, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sebastian Hagel, Bodo Saake
Feb 14, 2021·Marine Drugs·Antoine DecampOlivier Gonçalves
Dec 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Malte Jörn KrafftBodo Saake
Apr 17, 2021·Carbohydrate Polymers·Aleksandra Vojvodić CebinEstelle Bonnin
Apr 16, 2017·Journal of the American Society for Mass Spectrometry·Matthew T CampbellGary L Glish

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