A New Method for the Quantification of Rhizoctonia solani and R. oryzae from Soil

Plant Disease
T C Paulitz, Kurtis L Schroeder

Abstract

Rhizoctonia solani anastomosis group (AG) 8 and R. oryzae are important root pathogens on wheat and barley in the dryland production areas of the inland Pacific Northwest. R. solani AG-8 is difficult to isolate from root systems and quantify in soil because of slow growth and low population densities. However, both pathogens form extensive hyphal networks in the soil and can grow a considerable distance from a food base. A quantitative assay of active hyphae was developed, using wooden toothpicks as baits inserted into sample soils. After 2 days in soil, toothpicks were placed on a selective medium, and the numbers of colonies that grew after 24 h were counted under a dissecting microscope. R. solani and R. oryzae could be distinguished from other fungi based on hyphal morphology. This method was tested in natural soils amended with known inoculum densities of R. solani AG-8 and R. oryzae. Regressions were used to compare the inoculum density or toothpick colonization curves to a predicted curve based on the volume of the toothpicks. The slopes and y intercept of log-log transformed regressions did not differ from the predicted curves in most cases. This technique was used to assess the hyphal activity of R. solani AG-8 and R. ...Continue Reading

References

May 1, 2003·FEMS Microbiology Ecology·Kirsty HarrisKarl Ritz
Apr 1, 2002·Plant Disease·T C Paulitz
Jun 1, 1990·The New Phytologist·C A Gilligan

❮ Previous
Next ❯

Citations

Aug 8, 2012·World Journal of Microbiology & Biotechnology·Vidhi ChaudharyAshok Kumar Bhatnagar
Jun 1, 2015·Plant Disease·T N SpurlockW S Monfort
May 2, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Patricia Ann OkubaraKimberlee K Kidwell
Apr 1, 2017·Plant Disease·Olutoyosi O Ajayi-Oyetunde, Carl A Bradley
Jan 24, 2019·Plant Disease·Angel José Vieira BlancoMurillo Lobo Junior
May 1, 2015·Plant Disease·Dipak Sharma-PoudyalLindsey J du Toit
Dec 21, 2018·Journal of Microbiological Methods·Anne T Pollard, Patricia A Okubara
Apr 10, 2020·Journal of Microbiological Methods·Amy L Salamone, Patricia A Okubara
Dec 21, 2021·Frontiers in Plant Science·Christine Jade Dilla-ErmitaScot H Hulbert
Jan 6, 2022·Archives of Microbiology·Mostafa M El-SheekhAbdelgawad Y Elsadany

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.