A new method for the quantitation of propofol in human plasma: efficient solid-phase extraction and liquid chromatography/APCI-triple quadrupole mass spectrometry detection

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Lakshmikant BajpaiDonn M Dennis

Abstract

Propofol (2,6-diisopropyl phenol) is widely used for the induction and maintenance of anesthesia. Analyses of its pharmacokinetics require simple and sensitive methods for quantitation of propofol in human plasma. Previously reported HPLC and GC methods are limited by cumbersome extraction steps. We describe a novel method that combines sample preparation by solid-phase extraction (SPE) with hydrophilic-lipophilic balance cartridges and analysis with a sensitive LC-APCI-triple quadrupole mass spectrometry (MS/MS) method for better quantitation. The absolute recovery of the analyte was greater than 96%. The limit of quantification for propofol in plasma at a signal-to-noise ratio of 10 was 5 ng/ml. The precision of the assay yielded coefficients of variation ranging from 2.9 to 5.3% and an accuracies of 99-105%. Our method advances the quantitative analysis of propofol in human plasma by combining simple, rapid and efficient SPE with specific and sensitive quantitation by HPLC with APCI-MS/MS detection.

References

Jul 1, 1991·British Journal of Anaesthesia·B MarshG N Kenny
May 19, 1993·Journal of Chromatography·H Y Yu, J K Liau
Nov 8, 1996·Journal of Chromatography. B, Biomedical Applications·W ElbastJ L Brazier
Dec 13, 2000·Biomedical Chromatography : BMC·A L Dawidowicz, E Fornal

❮ Previous
Next ❯

Citations

Sep 27, 2006·Anesthesia and Analgesia·Timothy E MoreyDonn M Dennis
Jun 8, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sun Young LeeJongki Hong
Jul 22, 2005·Journal of Mass Spectrometry : JMS
Jun 26, 2010·Biopharmaceutics & Drug Disposition·Timothy E MoreyDonn M Dennis
Oct 19, 2010·Journal of Forensic Sciences·Richard J Levy
Jul 23, 2013·Journal of Pharmaceutical and Biomedical Analysis·Hee Seung KimMoon Kyo In
Jan 15, 2014·Analytical and Bioanalytical Chemistry·Fabio VaianoElisabetta Bertol
Sep 8, 2011·Biomedical Chromatography : BMC·Buyun Chen, Michael G Bartlett
Oct 25, 2013·Archives of Pharmacal Research·Eunyoung Han
Jan 6, 2012·Biomedical Microdevices·Chien-Chong HongPo-Hsiang Chang
May 24, 2005·Journal of the American Society for Mass Spectrometry·Lakshmikant BajpaiDonn M Dennis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.