A new method for visualization of gut mucosal cells, describing the enterochromaffin cell in the rat gastrointestinal tract

Scandinavian Journal of Gastroenterology
B I GustafssonHelge L Waldum

Abstract

Enterochromaffin (EC) cells in the gastrointestinal tract have an important function as regulators of secretion, motility and sensation. The EC cell has traditionally been described as bottle-shaped, with basally located stores of serotonin. Stimuli acting on the apical membrane trigger serotonin release, which in turn activates the subepithelial sensory nerve terminals. To better describe the appearance of EC cells, we developed a new method for visualization of mucosal cells. The stomach, small intestine and large intestine were excised from Sprague-Dawley rats and then fixed in formalin. The organs were everted and filled with pronase solution. Single cells and aggregates of formalin-fixed mucosal cells were collected by scraping the mucosa off the muscularis mucosa. EC cells were visualized by staining for immunoreactivity against serotonin. EC cells with luminal extensions and very long (up to 80 microM) basally located axon-like extensions, sometimes connecting to neuron-like structures, were found. Other EC cells had no or only short and blunt basal extensions. Dividing serotonin-containing EC cells were also seen. These findings could make an important contribution towards furthering our understanding of EC cell functio...Continue Reading

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Citations

Jun 10, 2009·Molecular and Cellular Biochemistry·Hitoshi DoiharaHiroyuki Ito
Jul 8, 2008·Purinergic Signalling·Fievos Leontiou Christofi
Mar 4, 2014·PloS One·Diego V BohórquezRodger A Liddle
Sep 3, 2011·International Journal of General Medicine·Kine GregersenArnold Berstad
May 9, 2008·Acta Physiologica·M Berner Hansen, A-B Witte
Aug 8, 2007·Journal of Neuroendocrinology·Ø HausoH L Waldum
Dec 9, 2016·Expert Review of Gastroenterology & Hepatology·Magdy El-SalhyJan Gunnar Hatlebakk
Jun 17, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Mark KiddRoswitha Pfragner
Apr 27, 2011·International Journal of Experimental Pathology·Ashok R GunawardeneCarolyn A Staton
May 19, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·R L BertrandP P Bertrand
Aug 16, 2017·World Journal of Gastroenterology : WJG·Magdy El-SalhyJan Gunnar Hatlebakk
Jun 11, 2016·Biomedical Reports·Magdy El-SalhyJan Gunnar Hatlebakk
Jul 1, 2020·Frontiers in Physiology·Yoko B WangAmanda J Page
Mar 5, 2021·Gastroenterology·Lai WeiSeungil Ro

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