A new method of antibody elution from red blood cells

S A Chan-Shu, O Blair


A method of antibody elution from red blood cells using xylene is described. It can be completed in 15 minutes after cell washing and requires no special equipment. For warm antibodies, the eluates prepared were more potent than those prepared by Rubin's ether method. It was as effective as Landsteiner and Miller's heat elution method in investigations of ABO hemolytic disease of the newborn. The clinical application of this method in the investigation of hemolytic disease of the newborn, delayed transfusion reaction and autoimmune hemolytic anemia was described.


Sep 27, 1984·The New England Journal of Medicine·S KleinmanD Goldfinger
Jan 1, 1994·Vox Sanguinis·A S TorloniM G Hill
Feb 10, 2012·Veterinary Clinical Pathology·Emmeline TanDarren Wood
Jan 1, 1984·Vox Sanguinis·S PanzerC Mueller-Eckhardt
Mar 1, 1996·Clinical and Laboratory Haematology·C K LinC P Ng

Related Concepts

Blood Group H Type 1 Antigen
Anemia, Hemolytic, Idiopathic Acquired
Duffy Blood Group System
Erythroblastosis, Fetal
Kell Blood-Group System
Study, Methodological
Antigen D, Rh Blood Group
Acute Hemolytic Transfusion Reaction

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