A new method of antibody elution from red blood cells

Transfusion
S A Chan-Shu, O Blair

Abstract

A method of antibody elution from red blood cells using xylene is described. It can be completed in 15 minutes after cell washing and requires no special equipment. For warm antibodies, the eluates prepared were more potent than those prepared by Rubin's ether method. It was as effective as Landsteiner and Miller's heat elution method in investigations of ABO hemolytic disease of the newborn. The clinical application of this method in the investigation of hemolytic disease of the newborn, delayed transfusion reaction and autoimmune hemolytic anemia was described.

Citations

Sep 27, 1984·The New England Journal of Medicine·S KleinmanD Goldfinger
Jan 1, 1994·Vox Sanguinis·A S TorloniM G Hill
Feb 10, 2012·Veterinary Clinical Pathology·Emmeline TanDarren Wood
Jan 1, 1984·Vox Sanguinis·S PanzerC Mueller-Eckhardt
Mar 1, 1996·Clinical and Laboratory Haematology·C K LinC P Ng

Related Concepts

Blood Group H Type 1 Antigen
Anemia, Hemolytic, Idiopathic Acquired
Duffy Blood Group System
Erythroblastosis, Fetal
Erythrocytes
Kell Blood-Group System
Study, Methodological
Antigen D, Rh Blood Group
Xylene
Acute Hemolytic Transfusion Reaction

Related Feeds

Blood Group Incompatibility

Blood group incompatibility between donor and recipient has been associated with poor transplant outcomes. Discover the latest research on blood group icompatibility here.

Autoimmune Hemolytic Anemia

Autoimmune hemolytic anemia (AIHA) occurs when antibodies directed against the person's own red blood cells (RBCs) cause them to burst (lyse), leading to an insufficient number of oxygen-carrying red blood cells in the circulation. Discover the latest research on AIHA here.