A new method of metabarcoding Microsporidia and their hosts reveals high levels of microsporidian infections in mosquitoes (Culicidae).

Molecular Ecology Resources
Artur TrzebnyMiroslawa Dabert

Abstract

DNA metabarcoding offers new perspectives, especially with regard to the high-throughput identification and diagnostics of pathogens. Microsporidia are an example of widely distributed, opportunistic and pathogenic microorganisms in which molecular identification is important for both environmental research and clinical diagnostics. We have developed a method for parallel detection of both microsporidian infection and the host species. We designed new primer sets: one specific for the classical Microsporidia (targeting the hypervariable V5 region of small subunit [ssu] rDNA), and a second one targeting a shortened fragment of the COI gene (standard metazoan DNA-barcode); both markers are well suited for next generation sequencing. Analysis of the ssu rDNA data set representing 607 microsporidian species (120 genera) indicated that the V5 region enables identification of >98% species in the data set (596/607). To test the method, we used microsporidians that infect mosquitoes in natural populations. Using mini-COI data, all field-collected mosquitoes were unambiguously assigned to seven species; among them almost 60% of specimens were positive for at least 11 different microsporidian species, including a new microsporidian ssu r...Continue Reading

References

May 1, 1970·Journal of Invertebrate Pathology·N E Alger, A H Undeen
Sep 1, 1995·The Journal of Eukaryotic Microbiology·M D BakerJ A Shadduck
May 1, 1995·Journal of Molecular Evolution·H B Nicholas, W H McClain
Oct 1, 1994·Clinical Microbiology Reviews·R WeberR L Owen
Jul 1, 1993·Nucleic Acids Research·J M NeefsR De Wachter
Apr 1, 1997·The Journal of Infectious Diseases·T van GoolJ Dankert
Apr 29, 1998·Advances in Parasitology·L M Weiss, C R Vossbrinck
Jan 8, 1999·Journal of Invertebrate Pathology·J V MaddoxC R Vossbrinck
Jul 13, 2000·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Z ZhangW Miller
May 23, 2001·Microbes and Infection·C Franzen, A Müller
Jul 13, 2002·Diseases of Aquatic Organisms·Shu-Jen TsaiChung-Hsiung Wang
Jul 24, 2002·Nucleic Acids Research·Kazutaka KatohTakashi Miyata
Aug 23, 2002·Nature·Bryony A P WilliamsT Martin Embley
Nov 1, 2002·Journal of Clinical Microbiology·D M WolkJ E Rosenblatt
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Apr 8, 2004·The Journal of Eukaryotic Microbiology·Charles R VossbrinckJames J Becnel
May 19, 2004·Trends in Parasitology·Caspar Franzen
Jul 12, 2005·Folia Parasitologica·Charles R Vossbrinck, Bettina A Debrunner-Vossbrinck
Jul 12, 2005·International Journal for Parasitology·Yanji Xu, Louis M Weiss
Oct 6, 2006·Diagnostic Microbiology and Infectious Disease·Jaco J VerweijLisette van Lieshout
Mar 16, 2007·Journal of Cell Science·Galina V BeznoussenkoAlexander A Mironov
Aug 22, 2007·Journal of Invertebrate Pathology·Wei-Fone HuangChung-Hsiung Wang
Sep 15, 2007·Journal of the American Mosquito Control Association·Theodore G Andreadis
Feb 7, 2008·The Journal of Eukaryotic Microbiology·George Kyei-PokuKees van Frankenhuyzen
Jun 24, 2008·Bioinformatics·Aleksandr MorgulisAlejandro A Schäffer

❮ Previous
Next ❯

Methods Mentioned

BETA
light
electron microscopy
PCR
amplicon sequencing
electrophoresis
PCRs

Software Mentioned

geneious
fastx
figtree
gephi
fastq
[UNK]
partitionfinder2
mrbayes
genedoc
usearch

Related Concepts

Related Feeds

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Related Papers

Journal of the American Mosquito Control Association
Theodore G Andreadis
The Journal of Eukaryotic Microbiology
Alex M Ardila-GarciaNaomi M Fast
International Journal for Parasitology
H Elizabeth McClymontJudith E Smith
© 2022 Meta ULC. All rights reserved