A new method, using cis-regulatory control, for blocking embryonic gene expression.

Developmental Biology
Joel Smith, Eric H Davidson

Abstract

Many genes, and particularly regulatory genes, are utilized multiple times in unrelated phases of development. For studies of gene function during embryogenesis, there is often need of a method for interfering with expression only at a specific developmental time or place. Here we show that in sea urchin embryos cis-regulatory control systems which operate only at specific times and places can be used to drive expression of short designed sequences targeting given primary transcripts, thereby effectively taking out the function of the target genes. The active sequences are designed to be complementary to intronic sequences of the primary transcript of the target genes. In this work, the target genes were the transcription factors alx1 and ets1, both required for skeletogenesis, and the regulatory drivers were from the sm30 and tbr genes. The sm30 gene is expressed only after skeletogenic cell ingression. When its regulatory apparatus was used as driver, the alx1 and ets1 repression constructs had the effect of preventing postgastrular skeletogenesis, while not interfering with earlier alx1 and ets1 function in promoting skeletogenic mesenchyme ingression. In contrast, repression constructs using the tbr driver, which is active ...Continue Reading

References

May 25, 2002·Developmental Biology·Paola OliveriEric H Davidson
Jul 12, 2002·Nature·Gregory J Hannon
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hamid Bolouri, Eric H Davidson
Jan 8, 2004·BMC Biotechnology·George Kotzamanis, Clare Huxley
Mar 4, 2005·The International Journal of Biochemistry & Cell Biology·Shintaro Imamura, Shuji Kishi
Nov 15, 2006·Developmental Biology·Meredith Howard-AshbyEric H Davidson
Jul 20, 2007·Proceedings of the National Academy of Sciences of the United States of America·Roger Revilla-i-DomingoEric H Davidson
Jul 28, 2007·BMC Biotechnology·Shizuyo SutouTakeshige Otoi
Apr 17, 2008·Proceedings of the National Academy of Sciences of the United States of America·Paola OliveriEric H Davidson

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Citations

Jun 10, 2010·Zoological Science·Yuka OkamitsuNaoaki Sakamoto
Dec 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eric H Davidson, Michael S Levine
Aug 4, 2010·Development Genes and Evolution·Hiroyuki KogaHiroshi Wada
Aug 21, 2013·Integrative and Comparative Biology·Antje H L FischerJoel Smith
Nov 17, 2009·Current Opinion in Genetics & Development·Eric H Davidson
Feb 16, 2011·Developmental Biology·Eric H Davidson
Jul 30, 2019·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Sorin Istrail

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