A new micro-assay for hyaluronidase activity

Clinica Chimica Acta; International Journal of Clinical Chemistry
S S ChenP Hoffman

Abstract

A new micro-method for the assay of hyaluronidase activity is described. The method utilizes chondroitin sulfate as a substrate. The degraded products of chondroitin sulfate by hyaluronidase are determined by modified disc gel electrophoresis. The products are first concentrated into a single band in acrylamide gel, and then the band is stained with cetylpyridinium chloride. The absorbance of the band is proportional to the amount of the degraded products. The hyaluronidase activity is therefore linearly related to the absorbance. This procedure represents a sensitive method for the assay of hyaluronidase activity in the serum and urine.

Citations

Apr 1, 1967·Archives of Biochemistry and Biophysics·M De Salegui, W Pigman
Sep 1, 1973·Clinical Biochemistry·S N NorthrupH D Brown
Feb 6, 1960·Nature·G M BERLYNE
Oct 1, 1962·Analytical Biochemistry·T BITTER, H M MUIR
Sep 23, 1961·Nature·P J KNUDSEN, J KOEFOED
Apr 1, 1948·Proceedings of the Society for Experimental Biology and Medicine·R L MAYERD STANTON

Related Concepts

Chondroitin Sulfate, Zinc Salt
Urine
Acrylamide
Cetylpyridinium Chloride
Hyaluronidase Activity
Electrophoresis, Disc
Wydase
Hyaluronidase
Colorimetry
Turbidimetry

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