PMID: 213699Jan 1, 1978Paper

A new microplate neutralization test for typing of herpes simplex virus

Microbiology and Immunology
A Tada, K Yoshino

Abstract

A microplate serum neutralization test for estimation of complement-requiring neutralizing (CRN) antibody was established as the first step for simplification of typing of herpes simplex virus (HSV). When guinea pigs were immunized with type 2 HSV, the late sera could mostly differentiate the types of HSV better than hyperimmune rabbit sera, the CRN titer against the heterologous type 1 HSV being much lower than the homologous titer. Sera of guinea pigs immunized with type 1 HSV showed about the same level of cross reaction against type 2 HSV as did rabbit antisera. Guinea pig sera having minimal levels of cross reaction were selected, and their high dilution (1:160) and complement were added to serial 10-fold dilutions of virus in the microplate titration of virus infectivity. Selective reduction of virus titer by either antiserum could determine the type of HSV. No equivocal intermediate case was found among a number of stock strains including many fresh isolates. The typing result coincided with that determined by a modification of Yang et al's method based on virus titers obtained with Vero and primary chick embryo cells. The typing based on plaquing in chick embryo cells sometimes failed to identify type 1 HSV.

References

Jan 1, 1977·Microbiology and Immunology·A TadaK Yoshino
Feb 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·J P YangN S Chen
Mar 1, 1969·The Journal of General Virology·M E Figueroa, W E Rawls
Oct 1, 1969·Proceedings of the Society for Experimental Biology and Medicine·A J NahmiasA Duffey
Oct 1, 1971·Proceedings of the Society for Experimental Biology and Medicine·A J NahmiasD Thies
Oct 1, 1972·The Journal of General Virology·T SavageJ W Heine
May 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·M Ito, A L Barron
May 1, 1974·Japanese Journal of Microbiology·T KawanaK Yoshino
Mar 13, 1964·Archiv für die gesamte Virusforschung·S TANIGUCHI, K YOSHINO

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Citations

Jan 1, 1993·Microbiology and Immunology·T GotoT Kawana
Jan 1, 1982·Microbiology and Immunology·K YoshinoK Abe
Jan 1, 1981·Microbiology and Immunology·K Yoshino, K Abe

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