A new model of chronic hapten-induced colitis in young rats

Journal of Pediatric Gastroenterology and Nutrition
Leo R FitzpatrickRobert N Cooney

Abstract

: Chronic models of inflammatory bowel disease are lacking in preadult rodents. The primary goal of our study was to develop a chronic model of hapten-induced intestinal inflammation and fibrosis in young rats. Second, we aimed to determine the profiles of key Th-1, Th-2, and Th-17 proinflammatory and profibrotic cytokines, during the progression of colitis in young rats. Chronic hapten-induced colitis was induced by the administration of intracolonic 2,4,6-trinitrobenzene sulfonic acid (TNBS) in young Wistar rats (postnatal days 23, 35, 48, and 59). After 1, 3, or 4 cycles of TNBS, rats were euthanized and the colons were removed for the measurement of macroscopic, histologic, and biochemical parameters of colitis. Young rats developed moderate to severe colitis in the distal colon, without significant morbidity or mortality. Macroscopic severity, histologic pathology, and colonic weights increased progressively with repeated TNBS administration. Cobblestone-like ulceration and fibrosis was evident in the colon, particularly after 4 cycles of TNBS. There was a unique cytokine pattern associated with colitis in young rats. Interleukin (IL)-12 and tumor necrosis factor (TNF)-alpha peaked during the earlier postnatal time points ...Continue Reading

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Citations

Oct 12, 2013·World Journal of Gastroenterology : WJG·Pinar AtacaOsman Yilmaz
Jul 14, 2012·Journal of Digestive Diseases·Mei Ying ZhuWei Xiong Chen
Apr 9, 2013·Pharmacology & Therapeutics·Giovanni DothelFabrizio De Ponti
Jul 28, 2013·International Reviews of Immunology·Leo R Fitzpatrick
Sep 23, 2016·Annals of Medicine and Surgery·Efstathios AntoniouEmmanouil Pikoulis
Oct 28, 2016·Apoptosis : an International Journal on Programmed Cell Death·Jian-An BaiQiyun Tang
Nov 13, 2015·Gut Pathogens·Janelle A JiminezRichard R E Uwiera
Sep 4, 2012·Cellular & Molecular Immunology·Shao-Bo YangPing-Chang Yang
Jun 14, 2012·The Journal of Pharmacology and Experimental Therapeutics·Leo R FitzpatrickAldo Ammendola
Feb 12, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Dileep G NairMichael G Blennerhassett
May 10, 2013·Journal of Immunoassay & Immunochemistry·Ning XuChang-zhu Jin

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